Impact of cigarette smoke and aerobic physical training on histological and molecular markers of prostate health in rats
| Autor: | N.J. dos Santos, Danilo Bianchini Baptista, Allice Santos Cruz Veras, Giovana Rampazzo Teixeira, A.R. Florido Neto, Hayley Hope Allyssa Thorpe, Patricia Monteiro Seraphim |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade Estadual de Campinas (UNICAMP), University of Guelph, Universidade de São Paulo (USP) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Medicine (General) Time Factors Physiology Biochemistry 0302 clinical medicine Prostate Smoke General Pharmacology Toxicology and Pharmaceutics Biology (General) Receptor Inhalation General Neuroscience General Medicine Immunohistochemistry Endurance exercise Androgen receptor medicine.anatomical_structure 030220 oncology & carcinogenesis Tumor necrosis factor alpha medicine.symptom Growth factors Research Article medicine.medical_specialty medicine.drug_class QH301-705.5 Immunology Biophysics BCL-2 Ocean Engineering Inflammation 03 medical and health sciences R5-920 Endurance training Internal medicine Physical Conditioning Animal medicine Animals Rats Wistar business.industry Cell Biology Androgen Rats Disease Models Animal 030104 developmental biology Endocrinology Apoptosis BAX business Biomarkers |
| Zdroj: | Brazilian Journal of Medical and Biological Research, Vol 53, Iss 5 (2020) Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Brazilian Journal of Medical and Biological Research, Volume: 53, Issue: 5, Article number: e9108, Published: 17 APR 2020 Brazilian Journal of Medical and Biological Research v.53 n.5 2020 Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
| ISSN: | 0100-879X |
| Popis: | Made available in DSpace on 2020-12-12T02:03:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01. Added 1 bitstream(s) on 2021-07-15T14:38:05Z : No. of bitstreams: 1 S0100-879X2020000500601.pdf: 1540567 bytes, checksum: 0c79c38e3def6b85df458128c8155752 (MD5) Recent evidence suggests that aerobic physical training may attenuate the deleterious effects of cancer risk factors, including smoking. We investigated the effects of cigarette smoke inhalation and aerobic physical training on the expression of steroid receptors and inflammatory and apoptotic proteins in the prostate. Forty male Wistar rats were distributed in four groups: control (CO), exercise (EXE), cigarette smoke exposure (CS), and cigarette smoke exposure with exercise (CS+EXE). For eight weeks, animals were repeatedly exposed to cigarette smoke for 30 min or performed aerobic physical training either with or without the cigarette smoke inhalation protocol. Following these experiments, we analyzed prostate epithelial morphology and prostatic expression of androgen (AR) and glucocorticoid receptors (GR), insulin-like growth factor (IGF-1), B-cell lymphoma-2 (BCL-2), BCL-2-associated X protein (BAX), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and nuclear factor-kappa B (NF-κB) via immunohistochemistry. Cigarette smoke exposure stimulated the expression of AR, IGF-1, BCL-2, and NF-κB while downregulating BAX, IL-6, and TNF-α labeling in the prostate. In contrast, aerobic physical training attenuated cigarette smoke-induced changes in AR, GR, IGF-1, BCL-2, IL-6, TNF-α, and NF-κB. This suggests that cigarette smoke stimulates inflammation and reduces apoptosis, culminating in increased prostatic epithelial and extracellular matrices, whereas physical training promoted beneficial effects towards maintaining normal prostate morphology and protein levels. Programa de Pós-Graduação em Ciências da Motricidade Universidade Estadual Paulista Departamento de Educação Física Universidade Estadual Paulista Faculdade de Ciências e Tecnologia UNESP Programa de Pós-Graduação em Biologia Celular e Estrutural Universidade de Campinas Department of Biomedical Sciences Ontario Veterinary College University of Guelph Departamento de Fisioterapia Faculdade de Ciências e Tecnologias Universidade Estadual Paulista Programa de Pós-Graduação em Fisiologia Universidade de São Paulo Programa de Pós-Graduação em Ciências da Motricidade Universidade Estadual Paulista Departamento de Educação Física Universidade Estadual Paulista Faculdade de Ciências e Tecnologia UNESP Departamento de Fisioterapia Faculdade de Ciências e Tecnologias Universidade Estadual Paulista |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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