Heat Shock-Induced, Caspase-3-Independent Rapid Breakdown of Akt and Consequent Alteration of Its Total Phosphorylation/Activity Level
| Autor: | Sung-Il Yang, Heung-Soo Choi, Hyo-Jung Mo, Hong-Chang Lee |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Activity level
Proto-Oncogene Proteins c-akt Biophysics Caspase 3 Protein Serine-Threonine Kinases Biochemistry Proto-Oncogene Proteins medicine Animals Phosphorylation Heat shock Molecular Biology Protein kinase B COS cells Chemistry Cell Biology Cell biology Electroporation Caspases Shock (circulatory) COS Cells medicine.symptom Heat-Shock Response |
| Zdroj: | Biochemical and Biophysical Research Communications. 276:702-706 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.2000.3524 |
| Popis: | The immediate effect of a 15-min heat shock was examined on the level and the activity of Akt. Following heat shock, the Akt level decreased by 15-70% in a temperature-dependent and phosphorylation status-independent manner. This decrease of Akt level was not prevented by caspase inhibitors. At 48 degrees C, the extent of the breakdown was so immense that the total phosphorylation/activity level of Akt was not increased over the control level, implying that the total cellular activity of Akt governed by the level and the molar activity does not necessarily undergo the ensuing change. |
| Databáze: | OpenAIRE |
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