Comparison of inducible nitric oxide synthase mRNA expression in different airway portions and association with nitric oxide parameters from patients with asthma
Autor: | Meitetsu Masawa, Kazuyuki Chibana, Taichi Shiobara, Yukiko Horigane, Akihiro Takemasa, Ryosuke Koike, Ryo Arai, Yasuo Shimizu, Yoshiki Ishii, Nobuhiko Uchida, Yusuke Nakamura, Yoko Sato, Taiji Watanabe |
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Rok vydání: | 2019 |
Předmět: |
Male
0301 basic medicine Pathology medicine.medical_specialty Respiratory System Immunology Nitric Oxide Synthase Type II Nitric Oxide Real-Time Polymerase Chain Reaction Nitric oxide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Bronchoscopy medicine Humans Immunology and Allergy RNA Messenger Retrospective Studies biology medicine.diagnostic_test business.industry Exhalation Middle Aged respiratory system Immunohistochemistry Asthma Respiratory Function Tests respiratory tract diseases Nitric oxide synthase 030104 developmental biology Real-time polymerase chain reaction Bronchoalveolar lavage Gene Expression Regulation 030228 respiratory system chemistry Organ Specificity Asthma and Rhinitis Exhaled nitric oxide biology.protein Original Article Female ORIGINAL ARTICLES business Biomarkers Immunostaining |
Zdroj: | Clinical and Experimental Allergy |
ISSN: | 1365-2222 0954-7894 |
Popis: | Background Fractional exhaled nitric oxide concentration (FeNO) is widely used to support diagnosis and monitoring of bronchial asthma (BA). Tsoukias and George proposed a two‐compartment model (2CM) for assessing the alveolar concentration of NO, referred to as CANO(2CM), while Condorelli et al proposed a model based on the trumpet shape of the airway tree and axial diffusion (TMAD), referred to as CANO(TMAD). In addition, Högman et al proposed non‐linear model, referred to as CANO(non‐linear). Objective We examined associations between the expression of inducible nitric oxide synthase (iNOS) mRNA in airway cells (ACs) by bronchoscopy and NO‐parameters calculated by the three methods and identified which of them accurately reflected expression of iNOS mRNA from different airway portions. Methods We retrospectively analysed data of 18 patients with stable, mild‐moderate asthma, including 10 steroid‐naïve BA (snBA) patients. Samples were obtained from airway brushings and bronchoalveolar lavage (BAL). Expressions of iNOS protein in tissue samples were evaluated by immunostaining. The iNOS mRNA in ACs was measured by qPCR. NO‐parameters calculated by the three methods above and evaluated whether they were associated with iNOS mRNA in ACs derived from proximal (2nd carina), distal (10‐15th) airways and alveolar regions. Results Immunostaining revealed expression of iNOS proteins mainly in epithelial cells in the airways, while it was mainly expressed in macrophages in the alveolar region in the snBA group. The iNOS mRNA expression was increased in both proximal and distal ACs in the snBA group compared with steroid‐treated BA group (stBA). CANO(2CM) negatively associated with FEV 1 (%predicted) and also associated with iNOS mRNA in distal ACs significantly. However, CANO(TMAD) and CANO(non‐linear) showed no correlation with lung function nor iNOS mRNA expression in any portions of ACs. Conclusions These results suggested that CANO(2CM) reflected distal airway inflammation in steroid‐naïve asthma. |
Databáze: | OpenAIRE |
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