Comparison of inducible nitric oxide synthase mRNA expression in different airway portions and association with nitric oxide parameters from patients with asthma

Autor: Meitetsu Masawa, Kazuyuki Chibana, Taichi Shiobara, Yukiko Horigane, Akihiro Takemasa, Ryosuke Koike, Ryo Arai, Yasuo Shimizu, Yoshiki Ishii, Nobuhiko Uchida, Yusuke Nakamura, Yoko Sato, Taiji Watanabe
Rok vydání: 2019
Předmět:
Male
0301 basic medicine
Pathology
medicine.medical_specialty
Respiratory System
Immunology
Nitric Oxide Synthase Type II
Nitric Oxide
Real-Time Polymerase Chain Reaction
Nitric oxide
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Bronchoscopy
medicine
Humans
Immunology and Allergy
RNA
Messenger
Retrospective Studies
biology
medicine.diagnostic_test
business.industry
Exhalation
Middle Aged
respiratory system
Immunohistochemistry
Asthma
Respiratory Function Tests
respiratory tract diseases
Nitric oxide synthase
030104 developmental biology
Real-time polymerase chain reaction
Bronchoalveolar lavage
Gene Expression Regulation
030228 respiratory system
chemistry
Organ Specificity
Asthma and Rhinitis
Exhaled nitric oxide
biology.protein
Original Article
Female
ORIGINAL ARTICLES
business
Biomarkers
Immunostaining
Zdroj: Clinical and Experimental Allergy
ISSN: 1365-2222
0954-7894
Popis: Background Fractional exhaled nitric oxide concentration (FeNO) is widely used to support diagnosis and monitoring of bronchial asthma (BA). Tsoukias and George proposed a two‐compartment model (2CM) for assessing the alveolar concentration of NO, referred to as CANO(2CM), while Condorelli et al proposed a model based on the trumpet shape of the airway tree and axial diffusion (TMAD), referred to as CANO(TMAD). In addition, Högman et al proposed non‐linear model, referred to as CANO(non‐linear). Objective We examined associations between the expression of inducible nitric oxide synthase (iNOS) mRNA in airway cells (ACs) by bronchoscopy and NO‐parameters calculated by the three methods and identified which of them accurately reflected expression of iNOS mRNA from different airway portions. Methods We retrospectively analysed data of 18 patients with stable, mild‐moderate asthma, including 10 steroid‐naïve BA (snBA) patients. Samples were obtained from airway brushings and bronchoalveolar lavage (BAL). Expressions of iNOS protein in tissue samples were evaluated by immunostaining. The iNOS mRNA in ACs was measured by qPCR. NO‐parameters calculated by the three methods above and evaluated whether they were associated with iNOS mRNA in ACs derived from proximal (2nd carina), distal (10‐15th) airways and alveolar regions. Results Immunostaining revealed expression of iNOS proteins mainly in epithelial cells in the airways, while it was mainly expressed in macrophages in the alveolar region in the snBA group. The iNOS mRNA expression was increased in both proximal and distal ACs in the snBA group compared with steroid‐treated BA group (stBA). CANO(2CM) negatively associated with FEV 1 (%predicted) and also associated with iNOS mRNA in distal ACs significantly. However, CANO(TMAD) and CANO(non‐linear) showed no correlation with lung function nor iNOS mRNA expression in any portions of ACs. Conclusions These results suggested that CANO(2CM) reflected distal airway inflammation in steroid‐naïve asthma.
Databáze: OpenAIRE
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