Genomic analysis of the first isolate of KPC-2-producing Klebsiella pneumoniae from Uruguay
| Autor: | Verónica Elizabeth Alvarez, Daniela Centrón, Antonio Galiana, Graciela Borthagaray, Josefina Campos, Carolina Márquez Villalba |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Klebsiella pneumoniae 030106 microbiology Immunology Biology ST258 Microbiology beta-Lactamases Ciencias Biológicas 03 medical and health sciences Biología Celular Microbiología Humans Immunology and Allergy Phylogeny Colistin Genomics KLEBSIELLA PNEUMONIAE biology.organism_classification Klebsiella Infections KPC 030104 developmental biology KPC-2 producing Klebsiella pneumoniae Urinary Tract Infections Uruguay CIENCIAS NATURALES Y EXACTAS Genome Bacterial Multilocus Sequence Typing |
| Zdroj: | Journal of Global Antimicrobial Resistance. 15:109-110 |
| ISSN: | 2213-7165 |
| Popis: | Objectives: Since KPC-2-producing Klebsiella pneumoniae are associated with successful dissemination of a major clone, defined as sequence type 258 (ST258), the aim of this study was to perform whole-genome sequencing (WGS) of the first colistin-resistant K. pneumoniae strain (Kpn666) carrying blaKPC-2 identified in Uruguay in 2011 in order to identify genomic and phylogenetic traits. Methods: WGS of strain Kpn666 isolated from an asymptomatic urinary tract infection was performed using Illumina MiSeq, and de novo assembly was performed using SPADES v.3.11. Contigs were re-ordered using the ST258 reference genome NJST258_1 (GenBank CP006923) and were oriented with the MAUVE Contig Mover. Twenty complete genomes of K. pneumoniae identified as ST258 using the Pasteur MLST site were downloaded from GenBank (May 2017). A maximum-likelihood tree was created using MEGA7 based on core single nucleotide polymorphisms (SNPs) from whole-genome alignment obtained with SNP sites (https://github.com/sanger-pathogens/snp-sites). Results: WGS analysis revealed a genome of 5 448 179 bp (5232 CDS, 108 RNAs). Phylogenetic analysis identified that Kpn666 belonged to clade I lineage of ST258. Further studies also identified IncR, IncFIB(K) and IncFII(K) plasmid replicons and 11 transferable associated antimicrobial resistance genes (ARGs) comprising four drug classes. The mgrB gene involved in colistin resistance was shown to be disrupted by insertion of an IS5-like element. Conclusions: The first isolate of KPC-2-producing K. pneumoniae detected in Uruguay was sequenced and the results confirm the ability of this bacterium to capture several ARGs. The KPC-2 carbapenemase in Uruguay is likely to have been introduced by the high-risk clone ST258. Fil: Alvarez, Verónica Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina Fil: Campos, Josefina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina Fil: Galiana, Antonio. Hospital Maciel Montevideo; Uruguay Fil: Borthagaray, Graciela. Universidad de la República. Facultad de Química; Uruguay Fil: Centron, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina Fil: Márquez Villalba, Carolina. Universidad de la República. Facultad de Química; Uruguay |
| Databáze: | OpenAIRE |
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