The strong induction of metallothionein gene following cadmium exposure transiently affects the expression of many genes in Eisenia fetida: A trade-off mechanism?

Autor: Sébastien Lemière, R. Leroux, F. Brulle, Alain Leprêtre, Guillaume Mitta, Franck Vandenbulcke
Přispěvatelé: Laboratoire d'Ecologie Numérique et d'Ecotoxicologie, Université de Lille, Sciences et Technologies-Centre National de la Recherche Scientifique (CNRS), Parasitologie fonctionnelle et évolutive [2003-2006] (PFE), Centre National de la Recherche Scientifique (CNRS)-Université de Perpignan Via Domitia (UPVD), Institut de Génétique Moléculaire de Montpellier (IGMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Département d'Astrophysique, de physique des Particules, de physique Nucléaire et de l'Instrumentation Associée (DAPNIA), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Université de Lille, Sciences et Technologies-Centre National de la Recherche Scientifique ( CNRS ), Parasitologie fonctionnelle et évolutive ( PFE ), Université de Perpignan Via Domitia ( UPVD ) -Centre National de la Recherche Scientifique ( CNRS ), Institut de Génétique Moléculaire de Montpellier ( IGMM ), Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ), Département d'Astrophysique, de physique des Particules, de physique Nucléaire et de l'Instrumentation Associée ( DAPNIA ), Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ), Université de Perpignan Via Domitia (UPVD)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2007
Předmět:
MESH : RNA
Messenger

Physiology
Health
Toxicology and Mutagenesis

010501 environmental sciences
Toxicology
01 natural sciences
Biochemistry
MESH : Soil Pollutants
Soil Pollutants
Metallothionein
MESH: Animals
MESH : Copper
MESH : Cadmium
Regulation of gene expression
0303 health sciences
MESH : Gene Expression Regulation
Effector
MESH: Cyclophilin A
[SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE]
MESH: Oligochaeta
General Medicine
MESH: Gene Expression Regulation
MESH: Copper
[ SDV.BID.EVO ] Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE]
MESH : Oligochaeta
Cyclophilin A
Cadmium
Eisenia fetida
MESH: Cadmium
MESH : Cyclophilin A
Biology
Superoxide dismutase
MESH: Gene Expression Profiling
03 medical and health sciences
Heat shock protein
Animals
RNA
Messenger

Oligochaeta
Protein kinase C
MESH: RNA
Messenger

030304 developmental biology
0105 earth and related environmental sciences
MESH: Soil Pollutants
Gene Expression Profiling
MESH : Gene Expression Profiling
MESH: Metallothionein
MESH: Biological Markers
Cell Biology
biology.organism_classification
MESH : Metallothionein
MESH : Biological Markers
Gene expression profiling
Gene Expression Regulation
13. Climate action
biology.protein
MESH : Animals
Biomarkers
Copper
Zdroj: Comparative Biochemistry and Physiology-Part C: Toxicology and Pharmacology
Comparative Biochemistry and Physiology-Part C: Toxicology and Pharmacology, Elsevier, 2007, 144 (4), pp.334-41. ⟨10.1016/j.cbpc.2006.10.007⟩
Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology / Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology
Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology / Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology, 2007, 144 (4), pp.334-41. 〈10.1016/j.cbpc.2006.10.007〉
Comparative Biochemistry and Physiology-Part C: Toxicology and Pharmacology, 2007, 144 (4), pp.334-41. ⟨10.1016/j.cbpc.2006.10.007⟩
ISSN: 1532-0456
DOI: 10.1016/j.cbpc.2006.10.007
Popis: Metal pollution causes disturbances at various levels of biological organization in most species. Important physiological functions could be affected in the exposed individuals and among the main physiological functions, immunity may provide one (or more) effector(s) whose expression can be directly affected by a metal exposure in various macroinvertebrates. Protein expressions were studied in order to test them as molecular biomarkers of metal exposure in Eisenia fetida. Selected effectors were calmodulin, heat shock proteins, superoxide dismutase, catalase, metallothionein, beta-adrenergic receptor kinase, pyruvate carboxylase, transcriptionally controlled tumor protein, protein kinase C, ubiquitin and cyclophilin-A. The level of expression of each gene was analysed in whole organism following exposures to cadmium in soil using real-time PCR. Metallothionein, transcriptionally controlled tumor protein and cyclophilin-A expression were also measured following copper exposures in soil because these genes seemed to be sensitive to copper. This work enabled to distinguish metallothionein and cyclophilin-A among the 15 selected effectors. A strong decrease of the number of transcripts was also detected for most effectors soon after the exposure to cadmium suggesting that a trade-off mechanism occurs.
Databáze: OpenAIRE