The strong induction of metallothionein gene following cadmium exposure transiently affects the expression of many genes in Eisenia fetida: A trade-off mechanism?
Autor: | Sébastien Lemière, R. Leroux, F. Brulle, Alain Leprêtre, Guillaume Mitta, Franck Vandenbulcke |
---|---|
Přispěvatelé: | Laboratoire d'Ecologie Numérique et d'Ecotoxicologie, Université de Lille, Sciences et Technologies-Centre National de la Recherche Scientifique (CNRS), Parasitologie fonctionnelle et évolutive [2003-2006] (PFE), Centre National de la Recherche Scientifique (CNRS)-Université de Perpignan Via Domitia (UPVD), Institut de Génétique Moléculaire de Montpellier (IGMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Département d'Astrophysique, de physique des Particules, de physique Nucléaire et de l'Instrumentation Associée (DAPNIA), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Université de Lille, Sciences et Technologies-Centre National de la Recherche Scientifique ( CNRS ), Parasitologie fonctionnelle et évolutive ( PFE ), Université de Perpignan Via Domitia ( UPVD ) -Centre National de la Recherche Scientifique ( CNRS ), Institut de Génétique Moléculaire de Montpellier ( IGMM ), Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ), Département d'Astrophysique, de physique des Particules, de physique Nucléaire et de l'Instrumentation Associée ( DAPNIA ), Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ), Université de Perpignan Via Domitia (UPVD)-Centre National de la Recherche Scientifique (CNRS) |
Rok vydání: | 2007 |
Předmět: |
MESH : RNA
Messenger Physiology Health Toxicology and Mutagenesis 010501 environmental sciences Toxicology 01 natural sciences Biochemistry MESH : Soil Pollutants Soil Pollutants Metallothionein MESH: Animals MESH : Copper MESH : Cadmium Regulation of gene expression 0303 health sciences MESH : Gene Expression Regulation Effector MESH: Cyclophilin A [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] MESH: Oligochaeta General Medicine MESH: Gene Expression Regulation MESH: Copper [ SDV.BID.EVO ] Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] MESH : Oligochaeta Cyclophilin A Cadmium Eisenia fetida MESH: Cadmium MESH : Cyclophilin A Biology Superoxide dismutase MESH: Gene Expression Profiling 03 medical and health sciences Heat shock protein Animals RNA Messenger Oligochaeta Protein kinase C MESH: RNA Messenger 030304 developmental biology 0105 earth and related environmental sciences MESH: Soil Pollutants Gene Expression Profiling MESH : Gene Expression Profiling MESH: Metallothionein MESH: Biological Markers Cell Biology biology.organism_classification MESH : Metallothionein MESH : Biological Markers Gene expression profiling Gene Expression Regulation 13. Climate action biology.protein MESH : Animals Biomarkers Copper |
Zdroj: | Comparative Biochemistry and Physiology-Part C: Toxicology and Pharmacology Comparative Biochemistry and Physiology-Part C: Toxicology and Pharmacology, Elsevier, 2007, 144 (4), pp.334-41. ⟨10.1016/j.cbpc.2006.10.007⟩ Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology / Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology / Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology, 2007, 144 (4), pp.334-41. 〈10.1016/j.cbpc.2006.10.007〉 Comparative Biochemistry and Physiology-Part C: Toxicology and Pharmacology, 2007, 144 (4), pp.334-41. ⟨10.1016/j.cbpc.2006.10.007⟩ |
ISSN: | 1532-0456 |
DOI: | 10.1016/j.cbpc.2006.10.007 |
Popis: | Metal pollution causes disturbances at various levels of biological organization in most species. Important physiological functions could be affected in the exposed individuals and among the main physiological functions, immunity may provide one (or more) effector(s) whose expression can be directly affected by a metal exposure in various macroinvertebrates. Protein expressions were studied in order to test them as molecular biomarkers of metal exposure in Eisenia fetida. Selected effectors were calmodulin, heat shock proteins, superoxide dismutase, catalase, metallothionein, beta-adrenergic receptor kinase, pyruvate carboxylase, transcriptionally controlled tumor protein, protein kinase C, ubiquitin and cyclophilin-A. The level of expression of each gene was analysed in whole organism following exposures to cadmium in soil using real-time PCR. Metallothionein, transcriptionally controlled tumor protein and cyclophilin-A expression were also measured following copper exposures in soil because these genes seemed to be sensitive to copper. This work enabled to distinguish metallothionein and cyclophilin-A among the 15 selected effectors. A strong decrease of the number of transcripts was also detected for most effectors soon after the exposure to cadmium suggesting that a trade-off mechanism occurs. |
Databáze: | OpenAIRE |
Externí odkaz: |