Correlation between oxidation of low density lipoproteins and prostacyclin synthesis in cultured smooth muscle cells
| Autor: | Bengt Ek, Lena Humble |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
medicine.medical_specialty
Antioxidant Thiobarbituric acid medicine.medical_treatment Biochemistry Muscle Smooth Vascular Prostacyclin synthase Lipid peroxidation Lipoxygenase chemistry.chemical_compound Malondialdehyde Internal medicine Hydroxyeicosatetraenoic Acids medicine Animals Masoprocol Butylated hydroxytoluene Lipoxygenase Inhibitors Lipoprotein oxidation Cells Cultured Pharmacology Dose-Response Relationship Drug biology Butylated Hydroxytoluene Thiobarbiturates Epoprostenol Lipoproteins LDL Endocrinology chemistry biology.protein lipids (amino acids peptides and proteins) Lipid Peroxidation Rabbits Oxidation-Reduction |
| Zdroj: | Biochemical Pharmacology. 41:695-699 |
| ISSN: | 0006-2952 |
| DOI: | 10.1016/0006-2952(91)90068-g |
| Popis: | The effect of antioxidants on the oxidation of low density lipoproteins in relation to prosta-cyclin synthesis was investigated in the prescence of rabbit smooth muscle cells (SMC) and Fe-containing culture medium. The lipid peroxidation of low density lipoproteins (LDL) assayed as thiobarbituric acid reactive substances was increased from 0.5 to 1.4 nmol malondialdehyde mL by the presence of smooth muscle cells. Two potent antioxidants, nordihydroguairetic acid (NDGA) and butylated hydroxytoluene (BHT), inhibited lipoprotein oxidation by ic 50 values of 0.2 and 0.8 μM, respectively. Inhibition of lipoprotein oxidation was associated with an increased prostacyclin synthesis by the SMC, the effect being more pronounced with nordihydroguairetic acid than with butylated hydroxytoluene. The stable metabolite of the lipid hydroperoxide, 15-hydroxyeicosatetraenoic acid (15-HETE), formed in the 15-lipoxygenase pathway was measured following antioxidant treatment and found to be eliminated or greatly reduced by both antioxidants. The results presented show that lipid hydroperoxides, formed as a consequence of lipoprotein oxidation and promoted by the smooth muscle cells through a lipoxygenase reaction, may regulate prostacyclin synthase, a process which may be influenced by the addition of antioxidants. |
| Databáze: | OpenAIRE |
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