TMC1 and TMC2 Are Components of the Mechanotransduction Channel in Hair Cells of the Mammalian Inner Ear
| Autor: | Kotaro Ishikawa, Yoshiyuki Kawashima, Geoffrey C. Horwitz, Gwenaëlle S. G. Géléoc, Kiyoto Kurima, Bifeng Pan, Andrew J. Griffith, Jeffrey R. Holt, Yukako Asai |
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| Rok vydání: | 2013 |
| Předmět: |
Patch-Clamp Techniques
Neuroscience(all) Cell Count Mice Transgenic In Vitro Techniques Biology Mechanotransduction Cellular Article Biophysical Phenomena Adenoviridae Membrane Potentials Mice 03 medical and health sciences 0302 clinical medicine Transduction Genetic Hair Cells Auditory Evoked Potentials Auditory Brain Stem medicine Animals Mechanotransduction Organ of Corti Cells Cultured Vestibular Hair Cell Cochlea 030304 developmental biology 0303 health sciences Dose-Response Relationship Drug General Neuroscience Age Factors Membrane Proteins Cell biology medicine.anatomical_structure Acoustic Stimulation Mutation Auditory Perception Calcium Mechanosensitive channels Hair cell Transduction (physiology) Tip link 030217 neurology & neurosurgery |
| Zdroj: | Neuron. 79(3):504-515 |
| ISSN: | 0896-6273 |
| DOI: | 10.1016/j.neuron.2013.06.019 |
| Popis: | SummarySensory transduction in auditory and vestibular hair cells requires expression of transmembrane channel-like (Tmc) 1 and 2 genes, but the function of these genes is unknown. To investigate the hypothesis that TMC1 and TMC2 proteins are components of the mechanosensitive ion channels that convert mechanical information into electrical signals, we recorded whole-cell and single-channel currents from mouse hair cells that expressed Tmc1, Tmc2, or mutant Tmc1. Cells that expressed Tmc2 had high calcium permeability and large single-channel currents, while cells with mutant Tmc1 had reduced calcium permeability and reduced single-channel currents. Cells that expressed Tmc1 and Tmc2 had a broad range of single-channel currents, suggesting multiple heteromeric assemblies of TMC subunits. The data demonstrate TMC1 and TMC2 are components of hair cell transduction channels and contribute to permeation properties. Gradients in TMC channel composition may also contribute to variation in sensory transduction along the tonotopic axis of the mammalian cochlea. |
| Databáze: | OpenAIRE |
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