A comparison of in situ and in vitro methods to estimate in vivo fermentable organic matter of forages in ruminants

Autor: S. Tamminga, Claude Poncet, J.M.J. Gosselink, J. P. Dulphy, John W. Cone
Přispěvatelé: ProdInra, Migration, Unité de Recherches sur les Herbivores (URH), Institut National de la Recherche Agronomique (INRA), Wageningen University and Research Centre (WUR)
Jazyk: angličtina
Rok vydání: 2004
Předmět:
In situ
degradability
Animal Nutrition
[SDV]Life Sciences [q-bio]
degradation characteristics
Plant Science
Tilley & Terry
dairy-cows
ComputingMilieux_MISCELLANEOUS
2. Zero hunger
chemistry.chemical_classification
0303 health sciences
cellulase
rumen
biology
Chemistry
04 agricultural and veterinary sciences
Diervoeding
[SDV] Life Sciences [q-bio]
Biochemistry
gas-production profiles
gas production
Forage
Cellulase
[INFO] Computer Science [cs]
Development
03 medical and health sciences
Rumen
Animal science
In vivo
fermentable organic matter
Dry matter
Organic matter
[INFO]Computer Science [cs]
030304 developmental biology
pepsin
grass-silage
0402 animal and dairy science
prediction
040201 dairy & animal science
nylon bag
ID - Voeding
Red Clover
digestibility
dry-matter
biology.protein
WIAS
Animal Science and Zoology
protein
Agronomy and Crop Science
Food Science
Zdroj: Netherlands Journal of Agricultural Science, 52(1), 29-45
Netherlands Journal of Agricultural Science 52 (2004) 1
NJAS-Wageningen Journal of Life Sciences
NJAS-Wageningen Journal of Life Sciences, Elsevier, 2004, 52 (1), pp.29-45
ISSN: 0028-2928
1573-5214
Popis: Several known in situ and in vitro methods were compared for their reliability for determining directly or indirectly in vivo fermentable organic matter (in vivo FOM) of forages in ruminants. Twelve forage types were used: fresh and conserved forms of lucerne, red clover, orchard grass and perennial ryegrass. Organic matter truly digested in the rumen which in our study was regarded as equivalent to in vivo FOM was determined in six cannulated sheep, using the flow markers %sup51;Cr-EDTA and sRu-Phenanthrolin. In vivo FOM was estimated directly from results of the in situ nylon bag technique using three cows, and from the results of three in vitro methods, and indirectly by calculating in vivo FOM using equations from the Dutch and French protein evaluation systems. The in vitro methods were an enzymatic technique using pepsin and cellulase, the method of Tilley & Terry and the gas production technique. In vivo FOM was best correlated (R² = 0.74; n = 12) with gas production after 20 hours of incubation. The correlation improved when fresh and conserved forages were considered separately (R² = 0.90; n = 12). Indirectly, in vivo FOM was well estimated from the results of the in situ , the gas production and the Tilley & Terry methods (R² = 0.760.80; n = 12). The accuracy of the direct and indirect in vivo FOM estimates was similar. However, the direct in vivo FOM estimate was a regression and the indirect estimate was a validation. In conclusion, in vivo FOM was best estimated indirectly using the equation from the Dutch protein evaluation system, whereas the estimate was more accurate with the in situ and the gas production techniques than when the other in vitro methods were used.
Databáze: OpenAIRE
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