Use of In Vivo Imaging System Technology in Leishmania major BALB/c Mouse Ear Infection Studies
| Autor: | Alicia Cawlfield, Dylan Nugent, Franklyn Ngundam, Kirk Butler, Brian Vesely, Diana Caridha, Tobin Rowland |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
BALB/c Mouse 030106 microbiology Ear infection Leishmaniasis Cutaneous Parasite load Microbiology 03 medical and health sciences Mice Cutaneous leishmaniasis parasitic diseases medicine Animals Leishmania major Otitis Psychodidae Luciferases Mice Inbred BALB C General Veterinary biology Dose-Response Relationship Drug Leishmaniasis medicine.disease biology.organism_classification Leishmania Infectious Diseases Insect Science Luminescent Measurements Parasitology |
| Zdroj: | Journal of medical entomology. 55(2) |
| ISSN: | 1938-2928 |
| Popis: | Novel treatments for cutaneous leishmaniasis (CL) are needed, due to current lack of effective universal treatments, increasing resistance among the parasite, and toxic effects or impracticality of the current therapeutics. Models of direct infection with high number of Leishmania parasites in the current research of CL involving the BALB/c mouse or Golden Syrian Hamster are considered not suitable for the assessment of antileishmanial drug efficacy because of the lack of disease similarities with humans. The saliva of the sand fly vector is known to affect the host response to infection by the Leishmania parasite. Here, we build upon a previous BALB/c model infected with luciferase-expressing Leishmania major parasites. In the present study, we infect the ear dermis instead of the foot pad or base of the tail, and compare multiple methods of infection, using parasites alone or mixed with either bites from uninfected sand flies (Phlebotomus duboscqi Diptera Psychodidae:Neveu-Lemaire) or salivary gland sonicate from sand flies. Our data show a dose-response of bioluminescent signal (which represents the parasite load at the infection site), dermal lesion development, and Leishmania Donovan Units in liver and spleen. This in vivo L. major ear infection model, once optimized, can be used for assessing the efficacy of drug compounds that have been determined as very effective in the other, high inoculum CL models. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|