Synergistic effects of miR-708-5p and miR-708-3p accelerate the progression of osteoporosis
| Autor: | Jian Cao, Hongyan Li, Yanhua Feng, Yuhong Wang, Shengnan Huang, Shan Zhao, Huaying Xu, Ruran Wang, Guoying Xu, Haoran Huang |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Medicine (General) Osteoporosis Biochemistry Pre-Clinical Research Report 03 medical and health sciences 0302 clinical medicine R5-920 Osteogenesis medicine Animals Humans Osteoblasts business.industry Biochemistry (medical) Cell Differentiation Cell Biology General Medicine differentiation medicine.disease miR-708-5p miR-708-3p Rats MicroRNAs 030104 developmental biology osteoclasts 030220 oncology & carcinogenesis Cancer research Female business Homeostasis |
| Zdroj: | Journal of International Medical Research, Vol 48 (2020) The Journal of International Medical Research |
| ISSN: | 1473-2300 |
| Popis: | Background Bone homeostasis is a tightly orchestrated process maintained by osteoblasts and osteoclasts, and a disruption of their steady-state equilibrium can lead to the occurrence of osteoporosis (OP). Methods We investigated the differential expression of micro (mi)RNAs in the bone tissues of a postmenopausal osteoporosis rat model induced by ovariectomy (OVX). Real-time PCR was used to verify the differentially expressed miRNAs in bone samples from OP patients and controls. The specific targets of two differentially expressed miRNAs in osteogenic or osteoclast differentiation were determined by bioinformatic prediction, and mRNA and protein detection. Results miR-708-5p and miR-708-3p were highly expressed in the bone tissue of OVX rats and OP patients. miR-708-5p negatively regulated osteoblast differentiation in bone marrow mesenchymal stem cells by targeting SMAD specific E3 ubiquitin protein ligase 2, while miR-708-3p positively regulated osteoclast differentiation in bone marrow monocytes by targeting cerebellar degeneration associated protein 1 antisense RNA. miR-708-5p and miR-708-3p were shown to originate from the same precursor miRNA and to have a synergistic effect on the development of osteoporosis with different temporal and spatial patterns. Conclusion Our findings provide a referential theoretical basis and targets for the prevention and treatment of osteoporosis. |
| Databáze: | OpenAIRE |
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