Investigation of canine visceral leishmaniasis in a non-endemic area in Brazil and the comparison of serological and molecular diagnostic tests
| Autor: | Ana Pérola Drulla Brandão, Anaiá da Paixão Sevá, Bruna Cristine Rodrigues, Helio Langoni, Roberto Mitsuyoshi Hiramoto, Mariana Zanchetta e Gava, Rodrigo Martins Soares, Paula Ferraz de Camargo Zanotto, Fernando Ferreira, Tatiana Jimenez-Villegas, Silvia Neri Godoy |
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| Přispěvatelé: | Universidade de São Paulo (USP), Instituto Chico Mendez de Conservação da Biodiversidade, Universidade Estadual Paulista (UNESP), Instituto Adolfo Lutz |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Veterinary medicine Concordance RC955-962 Antibodies Protozoan Seroprevalence Enzyme-Linked Immunosorbent Assay Culling Serology Dogs Cãoservação Program Seroepidemiologic Studies DPP®-Dual Path platform Arctic medicine. Tropical medicine ELISA EM ANIMAL Major Article medicine Dog Animals Non endemic Dog Diseases Leishmania infantum Pathology Molecular business.industry Zoonosis Diagnostic test medicine.disease Infectious Diseases Visceral leishmaniasis Leishmaniasis Visceral Parasitology ELISA business Brazil IFA |
| Zdroj: | Revista da Sociedade Brasileira de Medicina Tropical, Vol 54 (2021) Revista da Sociedade Brasileira de Medicina Tropical v.54 2021 Revista da Sociedade Brasileira de Medicina Tropical Sociedade Brasileira de Medicina Tropical (SBMT) instacron:SBMT Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Revista da Sociedade Brasileira de Medicina Tropical, Volume: 54, Article number: e0182-2021, Published: 06 SEP 2021 |
| ISSN: | 1678-9849 |
| Popis: | Made available in DSpace on 2022-05-01T09:30:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 INTRODUCTION: Visceral leishmaniasis (VL) is an important zoonosis in Brazil. Previous identification of parasitized dogs can also help prevent the disease in humans, even in non-endemic areas of the country. The Brazilian Ministry of Health recommends diagnosis in dogs using a DPP® (rapid test) as a screening test and an immunoenzymatic assay (ELISA) as a confirmatory test (DPP®+ELISA), and culling infected dogs as a legal control measure. However, the accuracy of these serological tests has been questioned. METHODS: VL in dogs was investigated in a non-endemic area of the São Paulo state for three consecutive years, and the performances of different diagnostic tests were compared. RESULTS: A total of 331 dog samples were collected in 2015, 373 in 2016, and 347 in 2017. The seroprevalence by DPP®+ELISA was 3.3, 3.2, and 0.3%, respectively, and by indirect immunofluorescence assay (IFA), it was 3.0, 5.6, and 5.5%, respectively. ELISA confirmed 18.4% of DPP® positive samples. The concordance between the IFA and DPP® was 83.9%. The concordance between IFA and DPP®+ELISA was 92.9%. A molecular diagnostic test (PCR) was performed in 63.2% of the seropositive samples, all of which were negative. CONCLUSIONS: In non-endemic areas, diagnostic tests in dogs should be carefully evaluated to avoid false results. Universidade de São Paulo Departamento de Veterinária Preventiva e Saúde Animal Universidade Estadual de Santa Cruz, Departamento de Ciências Agrárias e Ambientais, Ilhéus, BA, Brasil Instituto Chico Mendez de Conservação da Biodiversidade Universidade Estadual Paulista Júlio de Mesquita Filho Departamento de Higiene Veterinária e Saúde Pública Instituto Adolfo Lutz Universidade Estadual Paulista Júlio de Mesquita Filho Departamento de Higiene Veterinária e Saúde Pública |
| Databáze: | OpenAIRE |
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