Development of In Vitro Seedling Screening Method for Selection of Resistant Rice Against Bakanae Disease
| Autor: | Yong-Hwan Lee, Jin-Woo Park, Hyo Won Choi, Kyung Seok Park, Inn Shik Myung, Myeong Ji Lee, Se Weon Lee, Sung Taek Kim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
biology
Resistance Sowing food and beverages Plant Science Plant disease resistance biology.organism_classification Biochemistry lcsh:S1-972 Spore Agar plate Horticulture Seedling Bakanae Paddy field Cultivar Rice lcsh:Agriculture (General) Fusarium fujikuroi Agronomy and Crop Science Molecular Biology Biotechnology |
| Zdroj: | Research in Plant Disease, Vol 17, Iss 3, Pp 288-294 (2011) |
| ISSN: | 2233-9191 1598-2262 |
| Popis: | Rice bakanae disease, caused by the fungus Fusarium fujikuroi, is one of the most important rice diseases anddistributed widely in Asia. Resistance screening system in rice field had been established. However, theevaluation results of the system vary according to the environmental conditions when the test is conducted. Todevelop precise and rapid evaluation method of disease resistance of rice to bakanae disease, in vitro screeningsystem was attempted in this study. The six cultivars namely, ‘Nampyeongbyeo’, ‘Junambyeo’,‘Chucheongbyeo’, ‘Samcheonbyeo’, ‘Odaebyeo’ and ‘Hwasinbyeo’ were tested. They were planted onto MSagar medium (10 ml) in test tube (450 mm × φ30 mm) and incubated at 25oC and 28oC in growth chamberunder 12 hr light condition. Symptoms of over growth appeared a few days after seeding and then seedlingwere withered 2−3 weeks after over growth. The disease symptoms such as leaf dryness on top of rice wereappeared in the ‘Nampyeongbyeo’ from 28 days at the concentration of 105 spores/ml culturing at 28oC andthen withered completely 35 days after seeding. Whereas the other varieties withered entirely 19−23 daysafter seeding. Using the in vitro seedling screening method, 72 rice varieties were investigated to selectresistant cultivar. Finally, two resistant cultivars (‘Nampyeongbyeo’ and ‘Inwolbyeo’) and seven moderatelyresistant cultivars (‘Hwadongbyeo’, ‘Seokjeongbyeo’, ‘Samgwangbyeo’, ‘Sampyeongbyeo’, ‘Nonghobyeo’,‘Heukjinjubyeo’ and, ‘Joanbyeo’) were selected. If in vitro seedling screening method was used for evaluationof bakanae disease resistance, it would be completed within 35 days after sowing of rice seed. |
| Databáze: | OpenAIRE |
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