Quantitative rotational-echo double resonance for Carbon-13 spin clusters
| Autor: | Jacob Schaefer, Gary J. Patti, Miriam Sindelar, Shigeru Matsuoka, Sonal Bansal |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Carbon Isotopes Nuclear and High Energy Physics Magnetic Resonance Spectroscopy Nitrogen Isotopes Dephasing Carbon-13 Biophysics Resonance Condensed Matter Physics Biochemistry Article Amino acid Glutamine Crystallography chemistry Solid-state nuclear magnetic resonance Humans Asparagine Spin (physics) |
| Zdroj: | J Magn Reson |
| ISSN: | 1090-7807 |
| DOI: | 10.1016/j.jmr.2021.107043 |
| Popis: | By using only half of the total evolution time for dephasing pulses, C{N} rotational-echo double resonance (REDOR) for clusters of (13)C spins (RDX) results in the same universal REDOR behavior as observed for isolated (13)C-(15)N pairs. RDX combines Hahn echoes with solid echoes to suppress interference from scalar J couplings. This is crucial for long evolution times. The modified version (which we call RDX24) makes RDX quantitative for (13)C clusters. We apply this scheme to human embryonic kidney cells labeled in culture by L-[(13)C(5) - (15)N(2)]-glutamine. We quantitatively characterize three separate nitrogen isotopic enrichments for: (i) the alpha nitrogens of glutamine residues in proteins (including the residues of the five amino acids synthesized from glutamine); (ii) the alpha nitrogens of the five amino-acid residues synthesized from glucose, together with those of the nine essential amino acids added to the growth medium; and (iii) the side-chain nitrogens of glutamine (and of asparagine derived from glutamine). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect