Single-Cell Protein Profiling by Microdroplet Barcoding and Next-Generation Sequencing

Autor:	John R. Haliburton, Zev J. Gartner, Adam R. Abate, Samuel Kim
Rok vydání:	2021
Předmět:	Microfluidics Quantitative proteomics High-Throughput Nucleotide Sequencing Proteins DNA Computational biology Biology Proteomics DNA barcoding Article DNA sequencing Transcriptome chemistry.chemical_compound Single-cell analysis chemistry DNA Barcoding Taxonomic Multiplex Single-Cell Analysis
Zdroj:	Methods in Molecular Biology ISBN: 9781071617700 Methods Mol Biol
Popis:	DNA barcoding of individual cells combined with next-generation sequencing enables high-throughput parallel analysis of biomolecules at the single-cell level. Encoding protein identity with DNA barcoding of specific antibody binders achieves sequencing-based protein quantitation by converting protein signals into DNA signals. Here, we describe how to prepare DNA-barcoded antibodies and connect protein identities to cellular identities using droplet microfluidics. This approach allows for multiplex single-cell protein analysis compatible with single-cell transcriptomic and mutational profiling methods.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ebad7ca158f203376f64afe4430504cf https://doi.org/10.1007/978-1-0716-1771-7_7 Zobrazit plný text záznamu