Discrimination of agonist and antagonist forms of CXCL10 in biological samples
| Autor: | M. Schmolz, Stanislas Pol, L. Stephen, Matthew L. Albert, James Mapes, Armanda Casrouge, Vincent Mallet, C. Pfister, A. Bisiaux |
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| Přispěvatelé: | Immunobiologie des Cellules Dendritiques, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Myriad rules based medicine, CHU Rouen, Normandie Université (NU), Service d'hépatologie médicale [CHU Cochin], Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre d'Immunologie Humaine (CIH), The work was funded by grants obtained from L'Institut National du Cancer (INCA), the European Research Council (ERC) and a private donation from Caisse de Retraite et de Prévoyance des Clercs et Employés de Notaires (CRPCEN)., The authors would like to thank members of the Centre d'Immunology Humaine (CIH) and the Pôle Integré de Recherche Clinique (PIRC) Institut Pasteur, and the members of the Liver Unit, Hopital Cochin for support of this work., Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Cochin [AP-HP], Centre d'Immunologie Humaine ( CIH ) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Male
MESH: Inflammation MESH: Carcinoma Transitional Cell/urine MESH : Aged MESH : Hepatitis C Chronic/blood Immunoenzyme Techniques MESH: Protein Structure Tertiary 0302 clinical medicine MESH : Culture Media Conditioned/chemistry MESH: Chemokine CXCL10/analysis [ SDV.IMM ] Life Sciences [q-bio]/Immunology Protein Isoforms Multiplex MESH: Protein Isoforms/immunology MESH: Culture Media Conditioned/chemistry Aged 80 and over MESH: Recombinant Fusion Proteins/analysis 0303 health sciences MESH: Middle Aged Antibodies Monoclonal Atopic dermatitis MESH: Enzyme-Linked Immunosorbent Assay/methods MESH : Protein Isoforms/immunology Neoplasm Proteins 3. Good health MESH : Peptide Fragments/immunology MESH: Urinary Bladder Neoplasms/urine MESH : Antibodies Monoclonal/immunology bladder cancer MESH : Protein Structure Tertiary Agonist MESH: Chemokine CXCL10/immunology MESH: Protein Isoforms/analysis MESH: Hepatitis C Chronic/blood Recombinant Fusion Proteins Immunology MESH : Body Fluids/chemistry 03 medical and health sciences MESH: Peptide Fragments/immunology MESH : Immunoenzyme Techniques/methods MESH: Antibodies Monoclonal/immunology Humans MESH : Middle Aged MESH : Aged 80 and over MESH : Protein Processing Post-Translational Aged MESH : Inflammation MESH: Humans MESH : Humans MESH: Adult MESH: Peptide Fragments/analysis medicine.disease Peptide Fragments Protein Structure Tertiary Chemokine CXCL10 Urinary Bladder Neoplasms chemokines/monokines Culture Media Conditioned MESH: Dipeptidyl Peptidase 4/metabolism MESH : Recombinant Fusion Proteins/analysis MESH: Female Biomarkers MESH : Protein Isoforms/analysis hepatitis C virus MESH : Carcinoma Transitional Cell/urine Translational Studies MESH: Body Fluids/chemistry medicine.disease_cause MESH: Neoplasm Proteins/urine MESH: Aged 80 and over Immunology and Allergy MESH : Female BCG MESH : Biomarkers MESH: Aged Predictive marker MESH : Adult Middle Aged Body Fluids MESH : Neoplasm Proteins/urine MESH : Enzyme-Linked Immunosorbent Assay/methods MESH : Chemokine CXCL10/immunology Biomarker (medicine) [SDV.IMM]Life Sciences [q-bio]/Immunology Female Adult medicine.drug_class MESH : Male Dipeptidyl Peptidase 4 Hepatitis C virus Enzyme-Linked Immunosorbent Assay MESH : Chemokine CXCL10/analysis MESH : Dipeptidyl Peptidase 4/metabolism medicine MESH : Urinary Bladder Neoplasms/urine MESH : Peptide Fragments/analysis 030304 developmental biology Inflammation Carcinoma Transitional Cell business.industry MESH: Immunoenzyme Techniques/methods Cancer Hepatitis C Chronic MESH: Male Transplantation MESH: Protein Processing Post-Translational MESH: Biomarkers business Protein Processing Post-Translational 030215 immunology |
| Zdroj: | Clinical and Experimental Immunology Clinical and Experimental Immunology, Wiley, 2012, 167 (1), pp.137-148. ⟨10.1111/j.1365-2249.2011.04488.x⟩ Clinical and Experimental Immunology, 2012, 167 (1), pp.137-148. ⟨10.1111/j.1365-2249.2011.04488.x⟩ Clinical and Experimental Immunology, Wiley, 2012, 167 (1), pp.137-148. 〈10.1111/j.1365-2249.2011.04488.x〉 |
| ISSN: | 0009-9104 1365-2249 |
| DOI: | 10.1111/j.1365-2249.2011.04488.x⟩ |
| Popis: | Summary The ready access to commercially available multiplex assays and the importance of inflammation in disease pathogenesis has resulted in an abundance of studies aimed at identifying surrogate biomarkers for different clinically important questions. Establishing a link between a biomarker and disease pathogenesis, however, is quite complex, and in some instances this complexity is compounded by post-translational modifications and the use of immunoassays that do not always discriminate between the different forms of the same protein. Herein, we provide a detailed description of an assay system that has been established to discriminate the agonist form of CXCL10 from the NH2-terminal truncated form of the molecule generated by dipeptidylpeptidase IV (DPP4) cleavage. We demonstrate the utility of this assay system for monitoring agonist and antagonist forms of CXCL10 in culture supernatant, patient plasma and urine samples. Given the important role of CXCL10 in chronic inflammatory diseases and its suggested role as a predictive marker in managing patients with chronic hepatitis C, asthma, atopic dermatitis, transplantation, tuberculosis, kidney injury, cancer and other diseases, we believe that our method will be of general interest to the research and medical community. |
| Databáze: | OpenAIRE |
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