Do large pores in the glomerular capillary wall account for albuminuria in nephrotic states?
| Autor: | Kerryn A Greive, Julijana Vuchkova, Maria Koltun, Wayne D. Comper |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
Cell Membrane Permeability Physiology Kidney Glomerulus Ficoll Puromycin Aminonucleoside Fractional clearance Rats Sprague-Dawley medicine Albuminuria Animals Carbon Radioisotopes Chromatography Chemistry Glomerular permeability Albumin Capillaries Rats Glomerular capillary Disease Models Animal Immunoglobulin G Hydrodynamics Biophysics Nephrosis medicine.symptom Glomerular Filtration Rate |
| Zdroj: | American Journal of Physiology-Renal Physiology. 307:F525-F532 |
| ISSN: | 1522-1466 1931-857X |
| DOI: | 10.1152/ajprenal.00228.2014 |
| Popis: | Albuminuria in nephrotic states is thought to arise from the formation of large pores in the glomerular capillary wall as large hydrodynamic probes, like Ficoll, have increased fractional clearance. In the present study, we tested for large pore formation in a novel manner. We accounted for the rates of plasma elimination as determined for tritium-labeled tracers of uncharged polydisperse Ficoll (radii range: 35–85 Å) and two globular14C-labeled proteins, albumin (radius: 36 Å) and IgG (radius: 55 Å), in control and puromycin aminonucleoside nephrotic (PAN) Sprague-Dawley rats. The plasma elimination rates were then matched to the urinary excretion of these labeled materials ( n = 7). Albumin and IgG plasma retention rates were identical and far enhanced compared with the retention rates of inert transport markers of equivalent hydrodynamic radius; their elimination rate corresponded to the elimination of a 75-Å radius Ficoll ( n = 5) and >105-Å radius dextran ( n = 5). In PAN, they were eliminated as ∼36- and ∼55-Å radii Ficoll, respectively, equivalent to their hydrodynamic radii. In contrast, there was no comparable increase in the elimination rate of Ficoll in PAN. The total plasma clearance of Ficoll in control and PAN rats and the urinary clearance in PAN rats were essentially the same for all radii. On the other hand, the urinary clearance of >45-Å radii Ficoll in controls was considerably lower with increasing radii, demonstrating a postfiltration cellular uptake in controls, which, when inhibited in nephrotic states, would account for apparent large pore formation. |
| Databáze: | OpenAIRE |
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