Line Probe Assay in the Rapid Detection of Rifampin-resistant Mycobacterium tuberculosis Directly from Clinical Specimens
| Autor: | Alexandr V. Vasilyef, Elena Vyshnevskaya, Harry J. Marttila, Boris Vyshnevskiy, Matti K. Viljanen, Tatjana F. Otten, Hanna Soini |
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| Rok vydání: | 1999 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Tuberculosis Microbial Sensitivity Tests Drug resistance Polymerase Chain Reaction Sensitivity and Specificity Microbiology Mycobacterium tuberculosis medicine Humans Line Probe Assay Antibiotics Antitubercular Antibacterial agent General Immunology and Microbiology biology Drug Resistance Microbial General Medicine biology.organism_classification rpoB medicine.disease Virology Infectious Diseases Mycobacterium tuberculosis complex Reagent Kits Diagnostic Rifampin Rifampicin medicine.drug |
| Zdroj: | Scandinavian Journal of Infectious Diseases. 31:269-273 |
| ISSN: | 1651-1980 0036-5548 |
| Popis: | The sensitivity of a PCR-based line probe assay (Inno-LiPA Rif. TB Assay; Innogenetics NV Zwijndrecht, Belgium) was studied by using nested-PCR technique. A total of 75 specimens, representing various body locations from 70 suspected tuberculosis patients were obtained. LiPA yielded 30 Mycobacterium tuberculosis complex positive results (sensitivity 58.8%, compared with final diagnoses) whereas culture for M. tuberculosis was positive in 18 specimens (sensitivity 35.3%). Genotypic rifampin resistance testing by LiPA showed that 7 specimens contained rpoB mutations associated with RMP resistance, and sequencing data of the rpoB gene and LiPA patterns agreed in 29 of 30 M. tuberculosis positive specimens (96.7%). This indicates reliable performance, which makes the test suitable for the rapid determination of resistance to rifampin directly in clinical samples. However, the best results are obtained if LiPA is combined with conventional staining and culture methods. |
| Databáze: | OpenAIRE |
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