Oral delivery of foreign antigens by attenuated Salmonella: consequences of prior exposure to the vector strain
| Autor: | R. Davies, Stephen R. Attridge, J. T. Labrooy |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Lipopolysaccharides
Salmonella animal diseases Genetic Vectors Dose-Response Relationship Immunologic Administration Oral Vaccines Attenuated medicine.disease_cause Pilus Microbiology Mice Plasmid Escherichia coli medicine Animals Vector (molecular biology) Antigens Bacterial Mice Inbred BALB C General Veterinary General Immunology and Microbiology biology Escherichia coli Proteins Public Health Environmental and Occupational Health biochemical phenomena metabolism and nutrition biology.organism_classification Virology Enterobacteriaceae Infectious Diseases Pilin Antigens Surface Bacterial Vaccines Humoral immunity biology.protein bacteria Molecular Medicine Female Fimbriae Proteins |
| Zdroj: | Vaccine. 15:155-162 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/s0264-410x(96)00158-2 |
| Popis: | Several strains of Salmonella have been used as vectors for the delivery of Escherichia coli fimbrial proteins to the gut-associated lymphoid tissue (GALT) of the mouse. Plasmids carrying a complementing thyA+ gene, together with genes specifying synthesis of K88 or K99, were introduced into non-reverting thyA Salmonella mutants. The resulting constructs expressed the foreign pilin protein on their surfaces and, provided the vector was able to colonize the GALT, elicited strong serum responses to K88 or K99. These responses were dramatically impaired however, in recipients with pre-existing immunity to the vector strain. Mice initially infected with Salmonella stanley ca 4, 10 or 20 weeks prior to oral administration of S. stanley-K88 showed greatly reduced serum responses to K88 as determined by ELISA. The hypo-responsiveness seen in vector-primed mice could be largely overcome by changing the serotype of the strain subsequently used to deliver the foreign protein. |
| Databáze: | OpenAIRE |
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