The Malassezia Sympodialis Allergen Mala s 11 Induces Human Dendritic Cell Maturation, in Contrast to Its Human Homologue Manganese Superoxide Dismutase
| Autor: | Annika Scheynius, Monica Vilhelmsson, Reto Crameri, Arezou Zargari, Catharina Johansson, Gunilla Jacobsson-Ekman |
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| Rok vydání: | 2007 |
| Předmět: |
Antigens
Fungal Immunology Immunoglobulins Cross Reactions medicine.disease_cause Homology (biology) Microbiology Superoxide dismutase Allergen Antigens CD medicine Humans Immunology and Allergy CD40 Antigens Antigen-presenting cell Cells Cultured chemistry.chemical_classification Malassezia Membrane Glycoproteins integumentary system biology Superoxide Dismutase Dendritic Cells HLA-DR Antigens General Medicine Dendritic cell Allergens Flow Cytometry biology.organism_classification Recombinant Proteins Phenotype Enzyme chemistry Malassezia sympodialis B7-1 Antigen biology.protein Cytokines B7-2 Antigen |
| Zdroj: | International Archives of Allergy and Immunology. 143:155-162 |
| ISSN: | 1423-0097 1018-2438 |
| DOI: | 10.1159/000099082 |
| Popis: | Background: Recently, we identified a major Malassezia sympodialis allergen, Mala s 11, which displays a high degree of DNA sequence homology to human manganese superoxide dismutase (hMnSOD). In atopic eczema patients sensitized to M. sympodialis, hMnSOD can elicit eczematous reactions and positive skin prick tests, suggesting cross- reactivity to Mala s 11 based on molecular mimicry. The objective of the current study was to compare the influence of Mala s 11 and hMnSOD on human dendritic antigen-presenting cells. Methods: Monocyte-derived dendritic cells (MDDCs) from healthy blood donors were co-cultured with recombinant Mala s 11 (rMala s 11), recombinant hMnSOD (rhMnSOD), lipopolysaccharide or cultured in medium alone. Phenotypic changes were analysed using flow cytometry and allogeneic lymphocyte proliferation assays. Cytokine release into culture supernatants was investigated using cytometric bead array. Results: Whereas rhMnSOD did not affect the MDDC phenotype, rMala s 11 up-regulated the maturation marker CD83, the co-stimulatory molecules CD40, CD80, CD86 and HLA-DR to a similar extent as lipopolysaccharide. Furthermore, rMala s 11, but not rhMnSOD, induced significantly higher levels of TNF-α, IL-6, IL-8, IL-10 and IL-12p70 in the culture supernatants at 24 h in comparison with MDDCs cultured in medium alone. Finally, MDDCs pre-incubated with rMala s 11 induced a significantly higher proliferation of allogeneic CD14-depleted peripheral blood monocytes than MDDCs pre-incubated with rhMnSOD. Conclusion: Our results suggest that Mala s 11, but not hMnSOD, affects the immune response of healthy individuals through dendritic cell maturation and cytokine release. This indicates that dendritic cells possess the ability to distinguish between Mala s 11 and its human homologue MnSOD. |
| Databáze: | OpenAIRE |
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