Changes in the status of nucleolus during long-term culturing of human HeLa cells
| Autor: | T. I. Bulycheva, O. O. Zharskaya, Olga V. Zatsepina, A. A. Grigoryev |
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| Rok vydání: | 2008 |
| Předmět: |
Time Factors
biology medicine.drug_class Nucleolus Immunocytochemistry Cell Culture Techniques Antibodies Monoclonal General Medicine biology.organism_classification Monoclonal antibody Molecular biology General Biochemistry Genetics and Molecular Biology In vitro Cell biology HeLa Antigen Cell culture Apoptosis medicine Animals Humans Antigens Cell Nucleolus HeLa Cells |
| Zdroj: | Bulletin of experimental biology and medicine. 144(3) |
| ISSN: | 0007-4888 |
| Popis: | Changes in the immunocytochemical status of the nucleoli during long-term (6–8 months) in vitro culturing of HeLa (carcinoma of the cervix uteri) cells were described using new A3 monoclonal antibodies selectively reacting with human cell nucleoli. The appearance of cells with abnormal location of A3 antigen was paralleled by a significant increase of culture sensitivity to some external factors (protein synthesis inhibition and oxidative stress). The data indicate that location of one of the nucleolar antigens is an indicator of the qualitative status of HeLa cells in the culture. |
| Databáze: | OpenAIRE |
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