Extractability of polyphenols from black currant, red currant and gooseberry and their antioxidant activity
| Autor: | Mircea Dumitru Croitoru, Ruxandra Stefanescu, Erzsébet Domokos, Timea Ülkei, Erzsébet Fogarasi, Eszter Laczkó-Zöld, Andrea Komlósi, Erzsébet Varga, Ibolya Fülöp |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
DPPH
Flavonoid Quinic Acid Ribes Antioxidants General Biochemistry Genetics and Molecular Biology Anthocyanins chemistry.chemical_compound 0404 agricultural biotechnology Glucosides Picrates Benzothiazoles Food science Chromatography High Pressure Liquid General Environmental Science chemistry.chemical_classification ABTS Neochlorogenic acid biology Plant Extracts Biphenyl Compounds Polyphenols Free Radical Scavengers 04 agricultural and veterinary sciences biology.organism_classification 040401 food science Neurology chemistry Polyphenol Anthocyanin Red currant Luminescent Measurements Indicators and Reagents Quercetin Chlorogenic Acid Sulfonic Acids |
| Zdroj: | Acta Biologica Hungarica. 69:156-169 |
| ISSN: | 1588-256X 0236-5383 |
| DOI: | 10.1556/018.69.2018.2.5 |
| Popis: | In this study, we analyzed extracts of Ribes (black currant, red currant and gooseberry) fruits obtained with methanol, methanol 50% and water. For each extract total polyphenol content, total flavonoid content and total anthocyanin content was assessed. The antioxidant activity of extracts was evaluated by 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging capacity and by the photo-chemiluminescence (PCL) method. Identification and quantification of individual phenolic compounds was performed by means of high performance liquid chromatograph coupled with diode array detector (HPLC-DAD) analyses. From each fruit, best extraction of polyphenols was obtained with methanol 50%. In case of red currants and gooseberry there was no significant difference in flavonoids and anthocyanins extraction rate by the different extraction solvents. For black currants the methanol and methanol 50% extract presented the highest antioxidant activity. For red currants extracts with methanol 50% showed stronger antioxidant activity (IC50 = 5.71 mg/ml for DPPH, IC50 = 1.17 mg/ml for ABTS) than those with methanol or water. In case of gooseberry by the DPPH test the water extract proved to be the most active (IC50 = 5.9 mg/ml). In the PCL test black currants methanol 50% extract was over 6 times more powerful as the ones from red currants. In case of gooseberries, water extract presented the highest antioxidant activity (41.84 μmol AAE/g). In black currant cyanidin-3-glucoside was the major compound. Quercetin 3-O-glucoside was identified in each sample. From cinnamic acid derivatives neochlorogenic acid was present in black currants in the highest amount (356.33 μg/g). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink