Characterization and localization of dynein and myosins V and VI in the ovaries of queen bees
| Autor: | Karen Luciana Calábria, Salmen Foued Espindola, Carminda da Cruz-Landim, Karina Pavão Patrício, Marco Pablo Peixoto |
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| Rok vydání: | 2010 |
| Předmět: |
Dynein
Immunocytochemistry Blotting Western Myosin Type V macromolecular substances Biology Epitope Motor protein Epitopes Mice Oogenesis Western blot Microscopy Electron Transmission Myosin medicine Animals Cytoskeleton medicine.diagnostic_test Myosin Heavy Chains Ovary Antibodies Monoclonal Dyneins Cell Biology General Medicine Bees Molecular biology Immunohistochemistry Polyclonal antibodies biology.protein Electrophoresis Polyacrylamide Gel Female Rabbits Biomarkers |
| Zdroj: | Cell biology international. 34(10) |
| ISSN: | 1095-8355 |
| Popis: | The presence of myosin and dynein in the ovaries of both Apis mellifera and Scaptotrigona postica was investigated in extracts and in histological sections. In the ovary extracts, motor proteins, myosins V, VI and dynein were detected by Western blot. In histological sections, they were detected by immunocytochemistry, using a mouse monoclonal antibody against the intermediary chain of dynein and a rabbit polyclonal antibody against the myosin V head domain. The myosin VI tail domain was recognized by a pig polyclonal antibody. The results show that these molecular motors are expressed in the ovaries of both bee species with few differences in location and intensity, in regions where movement of substances is expected during oogenesis. The fact that antibodies against vertebrate proteins recognize proteins of bee species indicates that the specific epitopes are evolutionarily well preserved. |
| Databáze: | OpenAIRE |
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