Pseuderanthemum palatiferum (Nees) Radlk extract induces apoptosis via reactive oxygen species-mediated mitochondria-dependent pathway in A549 human lung cancer cells
| Autor: | Pennapa Chonpathompikunlert, Udomlak Kongprasom, Morakot Sroyraya, Supita Tanasawet, Somporn Sretrirutchai, Wanida Sukketsiri |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
A549 cell
chemistry.chemical_classification Membrane potential Reactive oxygen species Pseuderanthemum biology 010405 organic chemistry Chemistry Pharmaceutical Science respiratory system Mitochondrion biology.organism_classification 01 natural sciences Molecular biology 0104 chemical sciences 010404 medicinal & biomolecular chemistry Apoptosis Pseuderanthemum palatiferum Apoptosis Reactive oxygen species Mitochondria Lung cancer Pharmacology (medical) MTT assay Viability assay |
| Zdroj: | Tropical Journal of Pharmaceutical Research; Vol 18, No 2 (2019); 287-294 |
| ISSN: | 1596-5996 1596-9827 |
| Popis: | Purpose: To investigate the capacity of aqueous Pseuderanthemum paltiferum leaf extracts (PPA) to induce apoptosis in A549 human lung cancer cells and the possible mechanisms of action. Methods: Human lung cancer A549 cells were cultured in the presence of PPA (0 - 1000 μg/mL). Cell viability was assessed by MTT assay while morphological alterations in the cells were observed by Hoechst 33342/PI double staining. Intracellular reactive oxygen species (ROS) levels and subsequent changes of mitochondrial membrane potential were also investigated. Involvement of caspase-3 activation in the apoptotic pathway was determined. Results: PPA inhibited the growth of A549 cells in a concentration- and time-dependent manner. Major phenotypic apoptotic cell death was evidenced in microscopic images. Furthermore, treatment of A549 cells with PPA resulted in a significant increase in the production of ROS accompanied by attenuation of mitochondrial membrane potential, thus inducing the activation of caspase-3 activity ( p < 0.05). Conclusion: PPA exerts anti-cancer activity by suppression of cell viability and induction of ROSmediated mitochondrial dependent apoptosis in A549 cells, and may be a potential candidate for the development of a therapeutic agent for lung cancer. Keywords: Pseuderanthemum palatiferum , Apoptosis, Reactive oxygen species, Mitochondria, Lung cancer |
| Databáze: | OpenAIRE |
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