Identification of β-Sitosterol as in Vitro Anti-Inflammatory Constituent in Moringa oleifera
| Autor: | Pei-Chun Liao, Jie Chen, Ming-Chih Tsai, Kuang-Ping Hsu, Louis Kuoping Chao, Ming-Hoang Lai, Narumon Jeyashoke, Yueh-Hsiung Kuo, Xi-Jiang Yin, Chun-Xiang Li |
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| Rok vydání: | 2018 |
| Předmět: |
Keratinocytes
0301 basic medicine Cell Survival Surface Properties medicine.drug_class Drug Compounding Anti-Inflammatory Agents Inflammation Antioxidants Anti-inflammatory Cell Line Moringa Mice 03 medical and health sciences 0302 clinical medicine medicine Animals Humans Secretion Particle Size Moringa oleifera Chemistry Macrophages Phytosterol Caspase 1 General Chemistry Sitosterols In vitro 030104 developmental biology Solubility Biochemistry Cell culture 030220 oncology & carcinogenesis Cytokines Nanoparticles medicine.symptom Signal transduction General Agricultural and Biological Sciences Signal Transduction |
| Zdroj: | Journal of Agricultural and Food Chemistry. 66:10748-10759 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.8b04555 |
| Popis: | β-Sitosterol is a well known phytosterol in plants, but owing to its poor solubility in typical media, determining its cellular mechanisms has been proven to be difficult. In this study, we investigated the anti-inflammatory activity of β-sitosterol (BSS) isolated from Moringa oleifera in two cell lines. Over a dose range of 7.5 to 30 μM, BSS dispersed well in the medium as nanoparticles with diameters of 50 ± 5 nm and suppressed the secretion of inflammatory factors from keratinocytes and macrophages induced by PGN, TNF-α, or LPS, such as TNF-α, IL-1β, IL-6, IL-8, and ROS, separately. In addition, BSS significantly reduced the expression of NLRP3, a key component of NLRP3 inflammasomes, and inhibited the activation of caspase-1. There was partial inhibition of NF-κB in macrophages. This is the first study to report an increase in the solubility of nearly water-insoluble phytosterols via the formation of nanoparticles and to delineate the formulation's capacity to inhibit the signal transduction pathways of inflammation in macrophages. |
| Databáze: | OpenAIRE |
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