Quantification and characterization of mucosa-associated and intracellular Escherichia coli in inflammatory bowel disease
| Autor: | Tim Elliott, B N Hudspith, Liljana Petrovska, Michael B. Cooley, Jeremy D. Sanderson, Clifton K. Fagerquist, Robert E. Mandrell, Luke P. Randall, Alex Boussioutas, Guanghui Wu, Jonathan Brostoff, Gareth Parkes, Beatriz Quiñones, Neil B. Rayment |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
DNA Bacterial Male Proteome Virulence Factors Virulence Biology medicine.disease_cause Inflammatory bowel disease Bacterial Adhesion Microbiology Young Adult Intestinal mucosa Crohn Disease medicine Escherichia coli Immunology and Allergy Humans Colitis Intestinal Mucosa Cells Cultured Escherichia coli Infections Aged Aged 80 and over Crohn's disease Gastroenterology Middle Aged medicine.disease Prognosis Ulcerative colitis Case-Control Studies Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Colitis Ulcerative Female Caco-2 Cells Intracellular Biomarkers Follow-Up Studies |
| Zdroj: | Inflammatory bowel diseases. 19(11) |
| ISSN: | 1536-4844 |
| Popis: | BACKGROUND Mucosa-associated Escherichia coli are abundant in inflammatory bowel disease (IBD), but whether these bacteria gain intracellular access within the mucosa is uncertain. If E. coli does gain intracellular access, the contribution of bacterial pathogenicity to this requires further elucidation. This study aimed to quantify and characterize mucosa-associated and intracellular E. coli in patients with IBD and in healthy control subjects (HC). METHODS Mucosal biopsies from 30 patients with Crohn's disease (CD), 15 with ulcerative colitis (UC), and 14 HC were cultured with or without gentamicin protection to recover intracellular or mucosa-associated E. coli, respectively. Overall, 40 strains (CD: n = 24, UC: n = 9, and HC: n = 7) were characterized by phylogenetic typing, adhesion and invasion assays, detection of virulence factors, antimicrobial resistance genes, and proteomic analysis. RESULTS Mucosa-associated E. coli were more abundant in CD and UC than in HC (2750 versus 1350 versus 230 median colony-forming units per biopsy; P = 0.01). Intracellular E. coli were more prevalent in CD (90%) than in UC (47%) or HC mucosal biopsies (0%) (P < 0.001). Of 24 CD strains, 2 were adherent and invasive, but there were no unifying pathogenicity determinants that could distinguish most CD strains from UC or HC strains, or intracellular isolates from mucosa-associated isolates. CONCLUSIONS Intracellular E. coli are more common in CD than in UC and not identified in HC. Most intracellular E. coli did not have characterizing pathogenic features, suggesting a significant role for defects in mucosal immunity or barrier dysfunction in their ability to gain intracellular access. |
| Databáze: | OpenAIRE |
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