Inhibition of T cell/B cell interaction by B-CLL cells
| Autor: | Martin Wilhelm, Hans-Peter Tony, Anneliese Schimpl, Wohlleben G, Mehringer C, Martin Goller, Christian Kneitz |
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| Rok vydání: | 1999 |
| Předmět: |
Cancer Research
T-Lymphocytes T cell CD40 Ligand B-cell receptor Naive B cell Cell Communication Biology Transfection Immunophenotyping Antigens CD hemic and lymphatic diseases medicine Humans Cytotoxic T cell CD40 Antigens Antigen-presenting cell Cells Cultured B cell B-Lymphocytes HLA-D Antigens Membrane Glycoproteins ZAP70 Hematology Flow Cytometry Leukemia Lymphocytic Chronic B-Cell Clone Cells Cell biology B-1 cell Kinetics medicine.anatomical_structure Oncology Immunology Spleen |
| Zdroj: | Leukemia. 13:98-104 |
| ISSN: | 1476-5551 0887-6924 |
| DOI: | 10.1038/sj.leu.2401235 |
| Popis: | The course of disease in patients suffering from chronic lymphocytic leukemia (CLL) is determined by a profound dysregulation of the immune system. The resulting immune suppression is the main cause of death in those patients. In the present study we addressed the question of whether leukemic B cells (B-CLL) are able to suppress regular T cell/B cell interaction. Activated CD4 + T cell clones induce expression of the early activation antigen CD23 on B lymphocytes in vitro. Under conditions used, this B cell activation event was dependent upon direct T cell contact. Addition of certain bystander B-CLL cells or normal B lymphocytes resulted in a cell number-dependent inhibition of B cell induction. This seems to reflect the competition of B-CLL cells for a cell contact-mediated T cell helper signal. By using CD40 ligand transfected fibroblasts as a substitute for T cell help, we show that the same B-CLL cells also suppress CD40 ligand-mediated B cell activation. B-CLL cells differ in their ability to inhibit CD40 ligand-mediated B cell activation. Some B-CLL cases (eight out of 14) are unable to compete for the T cell or CD40 ligand-mediated signal, even though they can functionally interact with CD40 ligand and thereby get activated themselves. In addition, these results indicate that the observed inhibition is not a result of cell crowding by merely reducing the chance of specific B cell/T cell interactions. Collectively, these data indicate that B-CLL cells are able to inhibit the interaction of activated T lymphocytes with normal B lymphocytes in vitro. Perturbed T cell/B cell interaction may represent an important mechanism underlying the various defects of the specific immune system observed in patients suffering from B-CLL. |
| Databáze: | OpenAIRE |
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