Synthesis of Isomaltooligosaccharides (IMOs) from Sweet Potato Starch by Simultaneous Saccharification and Transglycosylation Using Saccharomyces cerevisiae Var. diastaticus BE 134 to Improve Purity of IMOs
| Autor: | Thu Trang Vu, Van Anh Nguyen Thi, Hong Nga Luong, Hong Gam Nguyen Thi, Quan Duong Hong, Thu Hien Nguyen Thi, Ngoc Hoa Nguyen, Truc Lam Nguyen Thi, Tri Hoang Minh |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
endocrine system Chromatography Article Subject biology Pullulanase Chemistry Nutrition. Foods and food supply Prebiotic medicine.medical_treatment Saccharomyces cerevisiae fungi Liquefaction Oligosaccharide biology.organism_classification Yeast Hydrolysis medicine TX341-641 Safety Risk Reliability and Quality Potato starch Food Science |
| Zdroj: | Journal of Food Quality, Vol 2021 (2021) |
| ISSN: | 1745-4557 0146-9428 |
| Popis: | This study developed a simple two-step procedure to produce isomaltooligosaccharides (IMOs) from low-cost sweet potato starch (SPS). Effect of various reaction parameters on the steps of the synthesis process of IMOs was systematically investigated. The results show that Spezyme Xtra enzyme was the most suitable for the liquefaction step. The oligosaccharide components’ contents, including G1–G10 and G2–G6, reached 73.95 ± 0.02% and 49.24 ± 3.19%, respectively, after liquefaction. The simultaneous saccharification and transglycosylation (SST) reaction of SPS followed the liquefaction after the α-amylase activity was deactivated. This reaction was simultaneously treated by β-amylase, pullulanase, and α-transglucosidase. The effect of various reaction parameters, consisting of solution pH, reaction temperature, enzyme dosage, and reaction time, on the SST reaction to synthesize IMOs from SPS was fully studied. The results showed that the highest concentration of IMOs (IG234) reached 68.85 ± 1.82 g/L at the optimal condition. The purification of pristine IMO was performed by adding Saccharomyces cerevisiae var. diastaticus BE 134 yeast cells at the final step of the procedure. In particular, the SST reaction for the synthesis of IMOs from SPS shortened SST reaction time by three times compared with other three-step synthesis procedures of IMOs. These findings show that the SPS-derived IMOs can be applied as a novel and inexpensive prebiotic healthcare product for human gastrointestinal health, dieters, and diabetics. |
| Databáze: | OpenAIRE |
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