The protective effects of DA-9801 (Dioscorea extract) on the peripheral nerves in streptozotocin-induced diabetic rats
| Autor: | Kyung Ae Lee, Heung Yong Jin, Hong Sun Baek, Tae Sun Park |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
Pain Threshold medicine.medical_specialty Kidney Cortex Renal cortex Medicine (miscellaneous) Nerve Tissue Proteins Streptozocin Diabetes Mellitus Experimental Rats Sprague-Dawley Random Allocation Diabetic Neuropathies Internal medicine Nerve Growth Factor Peripheral Nervous System Gastric mucosa medicine Animals Hot plate test Skin Nutrition and Dietetics biology business.industry Plant Extracts Anti-Inflammatory Agents Non-Steroidal Interleukin Streptozotocin Sciatic Nerve Surgery Rats medicine.anatomical_structure Nerve growth factor Endocrinology Neuroprotective Agents Gastric Mucosa Dietary Supplements biology.protein Cytokines Sciatic nerve Plant Preparations business medicine.drug Neurotrophin |
| Zdroj: | Journal of nutritional science and vitaminology. 59(5) |
| ISSN: | 1881-7742 |
| Popis: | It has been reported that DA-9801, an extract mixture of Dioscorea japonica Thunb and Dioscorea nipponica Makino, produces a neurotrophic activity. Therefore, this study was conducted to examine the neuroprotective effects of DA-9801 in streptozotocin-induced diabetic rats. The experimental rats were divided into six groups: the control group, Group I (non-diabetic rats treated with DA-9801), Group II (diabetic, non-treated rats) and Groups III, IV, and V (diabetic rats treated with DA-9801 at doses of 10, 50 or 100 mg/kg/d). Following a 16-wk course of oral treatment with DA-9801, functional parameters (von Frey filament test, hot plate test), biochemical parameters (nerve growth factor (NGF), tumor necrosis factor (TNF)-α, interleukin (IL)-6) were measured. An immunohistochemical staining was done to assess the neuroprotective effects of DA-9081 in the skin, sciatic nerve, gastric mucosa and renal cortex. In Week 8, pain was evoked by either tactile or thermal stimuli, whose threshold was significantly higher in Group III, IV and V than Group II. Western blot analysis showed a more significant increase in NGF and decrease in TNF-α and IL-6 in Group III, IV and V than in Group II (p |
| Databáze: | OpenAIRE |
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