| Autor: |
Ziya, Yurtal, Kadri, Kulualp, Hüseyin, Özkan, Serap, Cilaker Miçili, Halef, Doğan, Ali Rıza, Şişman, Nevin, Ersoy, Ceren, Kizmazoğlu, Akın, Yakan |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Popis: |
The aim of this study was to investigate the protective and therapeutic effects of bovineamniotic fluid(BAF) on the inhibition of epidural fibrosis (EF) after experimentallaminectomy.Forty female Sprague Dawley rats were used. The amniotic fluids were collected from each trimester of a pregnant cow. The rats were divided into 5 groups. Whereas no laminectomy was applied to the control group, animals in the sham group underwent laminectomy. Laminectomy was performed in the animals in other groups and the operation area was closed by dripping 1 mL of BAF collected in 3 trimesters of pregnancy. Animals were killed 28 days after the operation.Compared with control, VEGF gene expression levels were downregulated approximately 5-fold in BAF-2. Whereas IL-6 was upregulated approximately 8-fold in the sham, it was downregulated 5-fold and 3-fold in BAF-1 and BAF-2, respectively. There was downregulation in BAF-2 and BAF-3 in terms of CD105 gene expression levels.TGFβ1was upregulated approximately 2-fold in the sham group and downregulated in BAF-1 and BAF-2. Although histopathologic alterations including EF grade and fibroblast cell density were found to increase in the sham group, all BAFtreatmentdecreased those of alterations. The highest CD105immunoreactivitywas detected in the sham group. All BAF treatment markedly aggravated fibrosis via decreasing CD105 immunoreactivity. In terms of grading parameters, almost the closest grades to the control were determined in the BAF-2. BAF collected in thesecond trimesteris most effective in healing ofscar tissueand preventing fibrosis via decreasingmicrovesseland fibroblast densities.The results indicate that BAF may be used as a potential protective agent to prevent EF. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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