'Take It or Leave It'—Factors Regulating Competence Development and DNA Uptake in Campylobacter jejuni
| Autor: | Kerstin Stingl, Julia C. Golz |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
DNA
Bacterial QH301-705.5 Cell adaptation Campylobacter jejuni natural transformation Catalysis Neutralization Article Microbiology Inorganic Chemistry chemistry.chemical_compound Bacterial Proteins medicine Physical and Theoretical Chemistry Biology (General) Molecular Biology Pathogen QD1-999 Spectroscopy biology Chemiosmosis Organic Chemistry General Medicine genetic diversity biology.organism_classification Computer Science Applications Transformation (genetics) Chemistry medicine.anatomical_structure chemistry pH regulation Transformation Bacterial Bacteria DNA |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 18 International Journal of Molecular Sciences, Vol 22, Iss 10169, p 10169 (2021) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms221810169 |
| Popis: | Campylobacter jejuni has a large adaptive potential due to enormous genetic exchange. Factors regulating natural transformation in this food-borne pathogen are largely unknown but of interest for the application of sustained reduction strategies in the food-processing industry. Using a single cell DNA uptake assay, we visualized that recognition of methylated C. jejuni DNA was essential for the first step of DNA uptake into a DNase resistant state. Transformation rates using a resistance marker correlated with the fraction of competent bacteria, harboring one to maximally four locations of active DNA uptake, not necessarily being located at the cell pole. Competence developed with rising pH between 6.5 and 7.5 under microaerobic conditions and was nearly insensitive towards growth temperatures between 32 °C and 42 °C, CO2 concentrations ranging from 0 to 50% and growth rates. However, competence development was abolished at pH 5 or under aerobic stress conditions, in which the bacteria ceased growth but fully survived. The DNA uptake machinery in competent bacteria shut down at slightly acidic pH and was reversibly switched on upon neutralization. It was dependent on the proton motive force and, in contrast to competence development, slightly enhanced under aerobic conditions. The results suggest that natural transformation in C. jejuni occurs in the neutral and microaerobic intestinal environment for enhanced genetic diversity and pre-adaption before host switch. In addition, highly competent bacteria might be shed into the environment, still able to acquire genetic material for increased survival. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|