The OsmiR396c‐OsGRF4‐OsGIF1 regulatory module determines grain size and yield in rice
| Autor: | Jun Zhu, Qiming Deng, Xiuhong Zeng, Aiping Zheng, Wenbo Li, Ye Cao, Jing Zeng, Shiquan Wang, Ping Li, Lingxia Wang, Yun Ren, Huainian Liu, Shuangcheng Li, Fengyan Gao, Kailong Xie, Zhongshan He |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Edible Grain Quantitative Trait Loci Genetic network Locus (genetics) Plant Science Quantitative trait locus Oryza 01 natural sciences 03 medical and health sciences Grain weight Gene Regulatory Networks Research Articles Plant Proteins grain size growth‐regulating factor biology rice miR396 food and beverages biology.organism_classification Grain size 030104 developmental biology Agronomy Mutation Biophysics Grain yield Agronomy and Crop Science 010606 plant biology & botany Biotechnology Research Article GRF‐interacting factors |
| Zdroj: | Plant Biotechnology Journal |
| ISSN: | 1467-7652 1467-7644 |
| Popis: | Summary Grain weight is the most important component of rice yield and is mainly determined by grain size, which is generally controlled by quantitative trait loci (QTLs). Although numerous QTLs that regulate grain weight have been identified, the genetic network that controls grain size remains unclear. Herein, we report the cloning and functional analysis of a dominant QTL, grain length and width 2 (GLW2), which positively regulates grain weight by simultaneously increasing grain length and width. The GLW2 locus encodes OsGRF4 (growth‐regulating factor 4) and is regulated by the microRNA miR396c in vivo. The mutation in OsGRF4 perturbs the OsmiR396 target regulation of OsGRF4, generating a larger grain size and enhanced grain yield. We also demonstrate that OsGIF1 (GRF‐interacting factors 1) directly interacts with OsGRF4, and increasing its expression improves grain size. Our results suggest that the miR396c‐OsGRF4‐OsGIF1 regulatory module plays an important role in grain size determination and holds implications for rice yield improvement. |
| Databáze: | OpenAIRE |
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