Application of flow-cytometry in the study of apoptosis in neonatal rat cardiomyocytes
| Autor: | Igor Slivac, I. Malcic, Zlatko Kniewald, D. Polancec, Kristina Radošević, V. Gaurina Srček, Jasna Kniewald, M. Matijasic, Hrvoje Kniewald |
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| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_specialty
Programmed cell death Cell Survival Apoptosis Biology law.invention Flow cytometry Andrology Norepinephrine Confocal microscopy law Isoprenaline Internal medicine medicine Animals Myocyte Myocytes Cardiac Cells Cultured Cell Proliferation Fluorescent Dyes Analysis of Variance Microscopy Confocal Dose-Response Relationship Drug medicine.diagnostic_test Isoproterenol Carbocyanines Flow Cytometry Rats Dose–response relationship Endocrinology Animals Newborn neonatal cardiomyocytes isoproterenol norepinephrine apoptosis flow cytometry confocal microscopy Microscopy Electron Scanning Catecholamine Benzimidazoles medicine.drug |
| Zdroj: | Methods and Findings in Experimental and Clinical Pharmacology. 29:681 |
| ISSN: | 2013-0155 |
| DOI: | 10.1358/mf.2007.29.10.1147767 |
| Popis: | Depending on the concentration, catecholamines activate various intracellular signaling pathways and can induce apoptosis in cardiac myocytes. Although 5, 5’ , 6, 6’ -tetrachloro-1, 1’ , 3, 3’ -tetraethylbenzimidazolocarbocyanine iodide (JC-1) has been previously used to study mitochondria in intact cardiomyocytes, there have been no reports on the detection of apoptosis in neonatal cardiomyocytes in combination with flow cytometry and confocal microscopy. In our study, neonatal rat cardiomyocytes were exposed to norepinephrine (NE) and isoproterenol (ISO) in concentrations of 1 microM and 10 microM for 48 hours. NE concentrations of 1 microM and 10 microM decreased the number of viable cardiomyocytes by 18% (*p |
| Databáze: | OpenAIRE |
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