Establishment of a New Cell-Based Assay To Measure the Activity of Sweeteners in Fluorescent Food Extracts
| Autor: | Yasuka Toda, Shinji Okada, Takumi Misaka |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Taste
Photoprotein Aequorin aequorin Biosensing Techniques Article Cell Line Receptors G-Protein-Coupled Electron Transport Complex IV taste Taste receptor luminescence Humans sweet taste receptor Receptor Fluorescent Dyes photoprotein biology Chemistry General Chemistry Sweetness Fluorescence Biochemistry Sweetening Agents Luminescent Measurements biology.protein Cell-based assay fluorescence clytin-II General Agricultural and Biological Sciences Food Analysis Intracellular |
| Zdroj: | Journal of Agricultural and Food Chemistry |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf2029835 |
| Popis: | Taste receptors have been defined at the molecular level in the past decade, and cell-based assays have been developed using cultured cells heterologously expressing these receptors. The most popular approach to detecting the cellular response to a tastant is to measure changes in intracellular Ca(2+) concentration using Ca(2+)-sensitive fluorescent dyes. However, this method cannot be applied to food-derived samples that contain fluorescent substances. To establish an assay system that would be applicable to fluorescent samples, we tested the use of Ca(2+)-sensitive photoproteins, such as aequorin and mitochondrial clytin-II, as Ca(2+) indicators in a human sweet taste receptor assay. Using these systems, we successfully detected receptor activation in response to sweetener, even when fluorescent compounds coexisted. This luminescence-based assay will be a powerful tool to objectively evaluate the sweetness of food-derived samples even at an industry level. |
| Databáze: | OpenAIRE |
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