Identification of Key Functions Required for Production and Utilization of the Siderophore Piscibactin Encoded by the High-Pathogenicity Island irp-HPI in Vibrionaceae
| Autor: | Marta A. Lages, Lucía Ageitos, Jaime Rodríguez, Carlos Jiménez, Manuel L. Lemos, Miguel Balado |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | RUC. Repositorio da Universidade da Coruña Universitat Oberta de Catalunya (UOC) International Journal of Molecular Sciences; Volume 23; Issue 16; Pages: 8865 |
| Popis: | This article belongs to the Collection Microbial Virulence Factors [Abstract] Piscibactin is a widespread siderophore system present in many different bacteria, especially within the Vibrionaceae family. Previous works showed that most functions required for biosynthesis and transport of this siderophore are encoded by the high-pathogenicity island irp-HPI. In the present work, using Vibrio anguillarum as a model, we could identify additional key functions encoded by irp-HPI that are necessary for piscibactin production and transport and that have remained unknown. Allelic exchange mutagenesis, combined with cross-feeding bioassays and LC-MS analysis, were used to demonstrate that Irp4 protein is an essential component for piscibactin synthesis since it is the thioesterase required for nascent piscibactin be released from the NRPS Irp1. We also show that Irp8 is a MFS-type protein essential for piscibactin secretion. In addition, after passage through the outer membrane transporter FrpA, the completion of ferri-piscibactin internalization through the inner membrane would be achieved by the ABC-type transporter FrpBC. The expression of this transporter is coordinated with the expression of FrpA and with the genes encoding biosynthetic functions. Since piscibactin is a major virulence factor of some pathogenic vibrios, the elements of biosynthesis and transport described here could be additional interesting targets for the design of novel antimicrobials against these bacterial pathogens. M.B. was supported by grant PID2019-103891RJ-100 from MCIN/AEI/10.13039/501100011033 (Spain). The work was also supported by grants RTI2018-093634-B-C21/C22 from MCIN/AEI/10.13039/501100011033/FEDER “A way to make Europe”. Work in University of Santiago de Compostela and University of A Coruña was also supported by grants GRC2018/018 and GRC2018/039, respectively, from Xunta de Galicia and BLUEBIOLAB (0474_BLUEBIOLAB_1_E), Programme INTERREG V A of Spain-Portugal (POCTEP). L.A. also thanks Xunta de Galicia for a predoctoral fellowship co-funded with the European Social Fund (ED481A-2019/081) Xunta de Galicia; GRC2018/018 Xunta de Galicia; GRC2018/039 Xunta de Galicia; ED481A-2019/081 |
| Databáze: | OpenAIRE |
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