Studies on the Reaction of Dietary Methylglyoxal and Creatine during Simulated Gastrointestinal Digestion and in Human Volunteers
| Autor: | Stephanie Treibmann, Julia Groß, Susann Pätzold, Thomas Henle |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Glycation End Products
Advanced Nutrition and Dietetics Tandem Mass Spectrometry Lysine digestion gastrointestinal tract 1 2-dicarbonyl compounds methylglyoxal Maillard reaction glycation creatine urine metabolism Humans Digestion Arginine Creatine Magnesium Oxide Pyruvaldehyde Healthy Volunteers Food Science |
| Zdroj: | Nutrients; Volume 14; Issue 17; Pages: 3598 |
| ISSN: | 2072-6643 |
| DOI: | 10.3390/nu14173598 |
| Popis: | The reactive 1,2-dicarbonyl compound methylglyoxal (MGO) is consumed with food and its concentrations decrease during digestion. In the present paper, the reaction of MGO with creatine, arginine, and lysine during simulated digestion, and its reaction with creatine during the digestion in human volunteers, was studied. Therefore, simulated digestion experiments with a gastric and an intestinal phase were performed. Additionally, an intervention study with 12 subjects consuming MGO-containing Manuka honey and creatine simultaneously or separately was conducted. Derivatization with o-phenylenediamine and HPLC–UV was used to measure MGO, while creatine and glycated amino compounds were analyzed via HPLC–MS/MS. We show that MGO quickly reacts with creatine and arginine, but not lysine, during simulated digestion. Creatine reacts with 56% of MGO to form the hydroimidazolone MG-HCr, and arginine reacted with 4% of MGO to form the hydroimidazolone MG-H1. In the intervention study, urinary MG-HCr excretion is higher in subjects who consumed MGO and creatine simultaneously compared to subjects who ingested the substances separately. This demonstrates that the 1,2-dicarbonyl compound MGO reacts with amino compounds during human digestion, and glycated adducts are formed. These contribute to dietary glycation products consumed, and should be considered in studies investigating their physiological consequences. |
| Databáze: | OpenAIRE |
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