Possible oxidative effects of isotretinoin and modulatory effects of vitamins A and C in Saccharomyces cerevisiae
| Autor: | Silva Lemos Ala iacute de, Leopoldino Coelho Rodrigues Vanessa, Vin iacute cius Oliveira Marcus, Torequl Islam Md, Pablo Sousa de Aguiar Ra iacute, Maria Oliveira Ferreira da Mata Ana, Melo de Carvalho Ricardo, Fernanda Correia Jardim Paz M aacute rcia, Mendes Feitosa Chistiane, eacute lia de Carvalho Melo Ana, eacute lia Carvalho Melo Cavalcante Ana, eacute Carvalho Melo Cavalcante Ana |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Vitamin Antioxidant medicine.medical_treatment Assay isotretinoin vitamin A vitamin C oxidative stress Biology Pharmacology medicine.disease_cause Applied Microbiology and Biotechnology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo parasitic diseases Genetics medicine Molecular Biology Vitamin C In vitro toxicology Hypervitaminosis medicine.disease 030104 developmental biology chemistry Biochemistry 030220 oncology & carcinogenesis Trolox Agronomy and Crop Science Oxidative stress Biotechnology |
| Zdroj: | African Journal of Biotechnology; Vol 15, No 6 (2016); 145-152 |
| ISSN: | 1684-5315 |
| DOI: | 10.5897/ajb2015.15050 |
| Popis: | Isotretinoin (ITN), chemically known as 13-cis-retinoic acid, is a part of the broad group of compounds related to vitamin A. It is particularly employed in the treatment of nodular cystic acne and as an inhibitor of proliferation of neoplastic cells, by exerting a regulatory effect on the cell differentiation. This study aimed at investigating the possible oxidative effects of ITN and modulatory effects of vitamins A and C in mutant and non-mutant Saccharomyces cerevisiae strains. In addition, to reconfirm the oxidative effects, five in vitro antioxidant assays were also prepared taking the alpha-tocopherol analogue, trolox as a standard. In vivo study conducted on S. cerevisiae cells was carried out with ITN 20 µg/ml taking hydrogen peroxide (H2O2) as stressor (STR), whereas ITN 5 to 50 µg/ml was considered for in vitro assays taking similar dose of trolox (TRO). Results show ITN to have oxidative effect in both in vitro and in vivo tests. In conclusion, ITN produced oxidative effects and there may be an hypervitaminosis effect with vitamins A and C, thus insinuation to genetic material. Key words: Assay, isotretinoin, vitamin A, vitamin C, oxidative stress. |
| Databáze: | OpenAIRE |
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