A mass-differentiated library strategy for identification of sugar nucleotidyltransferase activities from cell lysates
| Autor: | Lin Liu, Rahman M. Mizanur, Joy M. Jackson, Nicola L. B. Pohl, Kwang-Seuk Ko |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
chemistry.chemical_classification
Spectrometry Mass Electrospray Ionization Molecular Structure Biophysics Virulence Cell Biology Carbohydrate Biology Nucleotidyltransferase medicine.disease_cause Nucleotide sugar Nucleotidyltransferases Biochemistry Small Molecule Libraries chemistry.chemical_compound Enzyme chemistry Escherichia coli medicine Carbohydrate Metabolism Sugar Molecular Biology Pathogen |
| Zdroj: | Analytical Biochemistry. 441:8-12 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/j.ab.2013.06.004 |
| Popis: | Sugar nucleotidyltransferases, or nucleotide sugar pyrophosphorylases, are ubiquitous enzymes whose activities have been correlated to disease states and pathogen virulence. Here we report a rapid "one-pot" method to identify a range of sugar nucleotidyltransferase activities of purified proteins or in cell lysates using a mass-differentiated carbohydrate library designed for mass spectrometry-based analysis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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