Use of a laser diffraction particle sizer for the measurement of mean diameter of multicellular tumor spheroids

Autor: Sandra Adam, Jill Daniel, C. D. Boothby, J. E. D. Dyson
Rok vydání: 1989
Předmět:
Zdroj: In Vitro Cellular & Developmental Biology. 25:946-950
ISSN: 1475-2689
0883-8364
DOI: 10.1007/bf02624008
Popis: Increasing use is being made of tumor cell lines cultured as cell aggregates (generally referred to as multicellular spheroids) in in vitro radiosensitivity and/or chemosensitivity tests. Conventional procedures for the determination of mean spheroid diameters for the construction of growth delay curves employ a microscope-image analyzer. However, this approach can prove excessively time consuming when large numbers of samples have to be measured. We have, therefore, been exploring the use of a Laser Diffraction Particle Sizer, the Malvern 2600 long bench model, for the measurement of mean spheroid diameter and size distribution. We report here a direct comparison between measurements carried out by the instrument and under the microscope. Also a comparison of growth curves for six cell lines constructed from measurements by the microscope and by the instrument. A number of factors that might affect the accuracy of spheroid diameter measurement by the instrument have been investigated: The effect of stirring to maintain the spheroids in suspension during measurement. Sampling error due to removal of a series of spheroid samples from culture flasks for measurement. Optimum numbers of scans to be carried out by the instrument to reach a constant value for mean diameter, and minimum SE of the mean.
Databáze: OpenAIRE
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