The assessment of metabolite alteration induced by –OH functionalized multi-walled carbon nanotubes in mice using NMR-based metabonomics
| Autor: | Ali Ramazani, Koorosh Fooladsaz, Yasamin Baghdadchi, Mojtaba Fathi, Ahmad Jalilvand, Maryam Khoshkam |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Medicine (General) Taurine QH301-705.5 Metabolite medicine.medical_treatment Pharmaceutical Science Multi-walled carbon nanotubes 02 engineering and technology General Biochemistry Genetics and Molecular Biology Steroid 03 medical and health sciences chemistry.chemical_compound R5-920 Metabolomics Metabolome medicine Biology (General) Chemometrics Original Research chemistry.chemical_classification Toxicity Lipid metabolism General Medicine 021001 nanoscience & nanotechnology Taurocholic acid NMR Amino acid 030104 developmental biology chemistry Biochemistry 0210 nano-technology |
| Zdroj: | BioImpacts : BI BioImpacts, Vol 8, Iss 2, Pp 107-116 (2018) |
| ISSN: | 2228-5652 2228-5660 |
| DOI: | 10.15171/bi.2018.13 |
| Popis: | Introduction: There is a fundamental need to characterize multiwalled carbon nanotubes (MWCNTs) toxicity to guarantee their safe application. Functionalized MWCNTs have recently attracted special interest in order to enhance biocompatibility. The aim of the current work was to study the underlying toxicity mechanism of the -OH-functionalized MWCNTs (MWCNTs-OH), using the powerful NMR-based metabonomics technique. Methods: Following intraperitoneal single-injection of mice with 3 doses of MWCNTs-OH and one control, samples were collected at four time points during 22-days for NMR, biochemistry, and histopathology analysis. Metabolome profiling and pathway analysis were implemented by chemometrics tools and metabolome databases. Results: Based on the 1H-NMR data, metabolic perturbation induced by MWCNTs-OH were characterized by altered levels of steroid hormones, including elevated androgens, estrogens, corticosterone, and aldosterone. Moreover, increased L-lysine, aminoadipate, taurine and taurocholic acid and decreased biotin were observed in the high-dose group (1 mg.kg-1 B.W.) compared to the control. The findings also indicated that steroid hormone biosynthesis, lysine biosynthesis, and biotin metabolism are the most affected pathways by MWCNTs-OH. Conclusion: These pathways can reflect perturbation of energy, amino acids, and fat metabolism, as well as oxidative stress. The data obtained by biochemistry, metabonomics, and histopathology were in good agreement, proving that MWCNTs-OH was excreted within 24 h, through the biliary pathway. |
| Databáze: | OpenAIRE |
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