Mutation of a family 8 glycosyltransferase gene alters cell wall carbohydrate composition and causes a humidity-sensitive semi-sterile dwarf phenotype in Arabidopsis
| Autor: | Sophie Kiang, George Coupland, Deborah Long, Nicholas C. Carpita, Douglas A. Shoue, Nga T. Lao, Tony A. Kavanagh |
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| Rok vydání: | 2004 |
| Předmět: |
DNA
Complementary DNA Plant Light Mutant Molecular Sequence Data Arabidopsis Carbohydrates Mutagenesis (molecular biology technique) Plant Science Gene Expression Regulation Enzymologic Plant Growth Regulators Cell Wall Gene Expression Regulation Plant Glycosyltransferase Genetics Arabidopsis thaliana Amino Acid Sequence Gene Phylogeny biology Base Sequence Sequence Homology Amino Acid Glycosyltransferase Gene Gene Expression Regulation Developmental Glycosyltransferases Humidity General Medicine Anther dehiscence biology.organism_classification Blotting Northern Neisseria gonorrhoeae Mutagenesis Insertional Fertility Phenotype Multigene Family Mutation biology.protein DNA Transposable Elements Agronomy and Crop Science |
| Zdroj: | Plant molecular biology. 53(5) |
| ISSN: | 0167-4412 |
| Popis: | The genome of Arabidopsis thaliana contains about 400 genes coding for glycosyltransferases, many of which are predicted to be involved in the synthesis and remodelling of cell wall components. We describe the isolation of a transposon-tagged mutant, parvus, which under low humidity conditions exhibits a severely dwarfed growth phenotype and failure of anther dehiscence resulting in semi-sterility. All aspects of the mutant phenotype were partially rescued by growth under high-humidity conditions, but not by the application of growth hormones or jasmonic acid. The mutation is caused by insertion of a maize Dissociation (Ds) element in a gene coding for a putative Golgi-localized glycosyltransferase belonging to family 8. Members of this family, originally identified on the basis of similarity to bacterial lipooligosaccharide glycosyltransferases, include enzymes known to be involved in the synthesis of bacterial and plant cell walls. Cell-wall carbohydrate analyses of the parvus mutant indicated reduced levels of rhamnogalacturonan I branching and alterations in the abundance of some xyloglucan linkages that may, however, be indirect consequences of the mutation. |
| Databáze: | OpenAIRE |
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