Site-specific augmentation of amnion cyclooxygenase-2 and decidua vera phospholipase-a2 expression in labor: Possible contribution of mechanical stretch and interleukin-1 to amnion prostaglandin synthesis

Autor: Shingo Fujii, Norimasa Sagawa, Masahiro Yoshida, Hiroaki Itoh, Kazuyo Kakui, Daizo Korita, Koichi Terakawa, Shigeo Yura
Rok vydání: 2002
Předmět:
medicine.medical_specialty
Blotting
Western
Gene Expression
Prostaglandin
Dinoprostone
Phospholipases A
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Western blot
Pregnancy
Fetal membrane
Internal medicine
Decidua
medicine
Humans
Amnion
RNA
Messenger
Prostaglandin E2
reproductive and urinary physiology
Fetus
Labor
Obstetric
030219 obstetrics & reproductive medicine
medicine.diagnostic_test
biology
Reverse Transcriptase Polymerase Chain Reaction
Membrane Proteins
Obstetrics and Gynecology
Biomechanical Phenomena
Isoenzymes
Phospholipases A2
medicine.anatomical_structure
Endocrinology
chemistry
Cyclooxygenase 2
Prostaglandin-Endoperoxide Synthases
embryonic structures
Cyclooxygenase 1
biology.protein
Female
Cyclooxygenase
Mechanoreceptors
030217 neurology & neurosurgery
Interleukin-1
medicine.drug
Zdroj: Journal of the Society for Gynecologic Investigation. 9:68-74
ISSN: 1071-5576
DOI: 10.1016/s1071-5576(01)00157-5
Popis: To investigate a possible site-specific augmentation of prostaglandin (PG) synthesis in the fetal membranes during labor. We used reverse transcriptase-polymerase chain reaction or Western blot analysis to evaluate the expression of cytosolic phopholipase A2 (cPLA2) and cyclooxygenase-1, -2 (COX-1, -2), in both the upper and lower parts of the amnion, chorion laeve, and decidua vera tissues from term pregnant women before (n = 8) and after labor (n = 24). Prostaglandin E2 (PGE2) secretion from amnion-derived WISH cells was assessed using enzyme-linked immunosorbent assay after stimulation by cyclic mechanical stretching and interleukin-1 (IL-1). The expression of cPLA2 and COX-1 and COX-2 mRNAs was detected in all samples examined. Western blot analysis revealed that COX-2 expression in the upper part of the amnion, chorion laeve, and decidua vera tissues after labor was 4.7-, 4.9-, and 3.7-fold higher than that before labor, respectively (P < .05 for all). The cPLA2 protein expression in the upper part of the amnion and chorion laeve tissues after labor was 14.0- and 8.8-fold higher than that before labor, respectively (P < .05 for both). Moreover, in specimens obtained after labor, the amnion COX-2 expression and the decidua vera cPLA2 expression in the lower part of the fetal membrane was 1.9- and 2.6-fold higher than the respective levels in the upper part (P < .05 for both). In an in vitor study, cyclic mechanical stretching significantly enhanced IL-1-augmented PGE2 secretion from WISH cells. In the lower part of the amnion and decidua vera tissues, adjacent to the dilating cervical canal, PG synthesis was upregulated site specifically after labor. Such enhancement of amnion PG synthesis might be regulated at least partly by IL-1 and cyclic distension.
Databáze: OpenAIRE
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