Pituitary adenylate cyclase-activating polypeptide (PACAP) 38 and PACAP27 differentially stimulate growth hormone release and mRNA accumulation in porcine somatotropes
| Autor: | Antonio J. Martínez-Fuentes, Justo P. Castaño, Francisco Gracia-Navarro, María M. Malagón, JoséC. Garrido-Gracia |
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| Rok vydání: | 1998 |
| Předmět: |
medicine.medical_specialty
Cell type Time Factors Somatotropic cell Swine Cell Adenylate kinase Cyclase General Biochemistry Genetics and Molecular Biology Immunoenzyme Techniques Pituitary Gland Anterior Internal medicine medicine Animals Secretion RNA Messenger General Pharmacology Toxicology and Pharmaceutics Cells Cultured In Situ Hybridization Messenger RNA Neurotransmitter Agents Chemistry Neuropeptides General Medicine Immunohistochemistry Stimulation Chemical medicine.anatomical_structure Endocrinology Growth Hormone Pituitary Adenylate Cyclase-Activating Polypeptide Female hormones hormone substitutes and hormone antagonists Intracellular |
| Zdroj: | Life sciences. 62(26) |
| ISSN: | 0024-3205 |
| Popis: | Pituitary adenylate cyclase-activating polypeptide (PACAP) has been suggested to regulate growth hormone (GH) secretion in several species. Here, we analyzed the in vitro effects of PACAP38 and PACAP27 on the secretory activity of porcine somatotropes. Cultures of porcine pituitary cells were treated with PACAP38 and PACAP27, and GH release, intracellular GH content, and GH mRNA levels were evaluated. Also, the time course of changes in the somatotrope content of GH and its mRNA in response to PACAPs were measured. Both PACAPs stimulated GH release from porcine somatotropes in a broad range of doses (10 −10 –10 −6 M), yet only PACAP27 elicited a dose-dependent response. GH cell content remained essentially unchanged after PACAP treatment. In contrast, both PACAPs induced significant and sustained increases in GH mRNA cell content, although the response to PACAP27 appeared faster (8 h) than to PACAP38 (16 h). These results demonstrate that PACAP stimulates GH production in porcine somatotropes. Furthermore, the differential responses induced by PACAP38 and PACAP27 suggest that distinct mechanisms mediate their effects on this cell type. |
| Databáze: | OpenAIRE |
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