Use of hematopoietic progenitor cell count on the Sysmex XE-2100 for peripheral blood stem cell harvest monitoring
| Autor: | Véronique Baccini, Loïc Garçon, Christophe Marzac, Liliane Dal Cortivo, Florence Ajchenbaum-Cymbalista, Sylvie Tondeur, Françoise Audat, Françoise Valensi, Rémi Letestu, François Lefrère, Ramdane Belhocine, Jean-Yves Perrot |
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| Rok vydání: | 2007 |
| Předmět: |
Cancer Research
Sysmex XE-2100 medicine.diagnostic_test business.industry Cell CD34 Antigens CD34 Hematology Hematopoietic Stem Cells Sensitivity and Specificity Blood Cell Count Flow cytometry Andrology Apheresis medicine.anatomical_structure Hematopoietic progenitor Oncology Immunology Tissue and Organ Harvesting medicine Enumeration Humans Stem cell business |
| Zdroj: | Leukemia & Lymphoma. 48:89-96 |
| ISSN: | 1029-2403 1042-8194 |
| DOI: | 10.1080/10428190600886149 |
| Popis: | Successful peripheral blood stem cell (PBSC) collection depends on the timing of apheresis based on CD34+ cell enumeration. Because this analysis is expensive and induces organization difficulties, we evaluated hematopoietic progenitor cell (HPC) quantification on the Sysmex XE-2100 as a surrogate analysis. We tested 157 blood samples for CD34+ cells and HPC counts. We found a good linear correlation between HPC and CD34+ and determined simple rules allowing to use HPC count in daily practice. We set a positive cut-off30 HPC/mm(3) for allowing PBSC harvest and a negative cut-off at 0 HPC/mm(3) for which collection should be delayed. These two situations accounted for 62% of cases and CD34+ cell count by flow cytometry confirmed HPC result in 95% of cases. Between 0 and 30 HPC/mm3, CD34+ enumeration is required for decision-making. We conclude that HPC count may be a useful surrogate for CD34+ enumeration in PBSC harvest monitoring. |
| Databáze: | OpenAIRE |
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