Crosstalk between the RNA Methylation and Histone-Binding Activities of MePCE Regulates P-TEFb Activation on Chromatin
| Autor: | Nakul M. Shah, Blerta Xhemalce, Sravan K. Devanathan, Samantha B. Shelton, Nathan S. Abell, Marvin Mercado |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
7SK RNA methylation non-coding RNA RNA polymerase II MePCE Methylation General Biochemistry Genetics and Molecular Biology Cell Line Histones Histone H4 03 medical and health sciences Humans Positive Transcriptional Elongation Factor B lcsh:QH301-705.5 Histone binding biology Chemistry RNA 7SK Small Nuclear RNA Methyltransferases Receptor Cross-Talk pTEFb Chromatin Cell biology 030104 developmental biology BCDIN3 lcsh:Biology (General) Gene Expression Regulation RNAP II pausing RNA methyltransferase activity biology.protein |
| Zdroj: | Cell Reports, Vol 22, Iss 6, Pp 1374-1383 (2018) |
| ISSN: | 2211-1247 |
| DOI: | 10.1016/j.celrep.2018.01.028 |
| Popis: | Summary: RNAP II switching from the paused to the productive transcription elongation state is a pivotal regulatory step that requires specific phosphorylations catalyzed by the P-TEFb kinase. Nucleosolic P-TEFb activity is inhibited by its interaction with the ribonuclear protein complex built around the 7SK small nuclear RNA (7SK snRNP). MePCE is the RNA methyltransferase that methylates and stabilizes 7SK in the nucleosol. Here, we report that MePCE also binds chromatin through the histone H4 tail to serve as a P-TEFb activator at specific genes important for cellular identity. Notably, this histone binding abolishes MePCE’s RNA methyltransferase activity toward 7SK, which explains why MePCE-bound P-TEFb on chromatin may not be associated with the full 7SK snRNP and is competent for RNAP II activation. Overall, our results suggest that crosstalk between the histone-binding and RNA methylation activities of MePCE regulates P-TEFb activation on chromatin in a 7SK- and Brd4-independent manner. |
| Databáze: | OpenAIRE |
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