In vitro uptake and metabolism of testosterone by goldfish, Carassius auratus, mesenteric adipose tissue
| Autor: | Paul M Rosenblum, Donitia A Sutton, Rebecca M Moore |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Time Factors
Adipose tissue Ether Biology chemistry.chemical_compound Endocrinology Goldfish Animals Mesentery Testosterone Enzyme Inhibitors Incubation Androstenols Aromatase Inhibitors Hydrolysis Extraction (chemistry) Water Metabolism Thin-layer chromatography In vitro Adipose Tissue chemistry Biochemistry Animal Science and Zoology Acid hydrolysis Chromatography Thin Layer |
| Zdroj: | General and Comparative Endocrinology. 127:190-197 |
| ISSN: | 0016-6480 |
| Popis: | Mesenteric adipose tissue was removed from adult goldfish, Carassius auratus, and incubated in vitro with (1α,2α)-3[H]testosterone (T). Total radioactivity in the medium decreased and tissue total radioactivity increased in a time-dependent fashion between 1 and 6 h of incubation with maximum uptake occurring between 4 and 6 h. After ether extraction and thin layer chromatography the amount of radioactivity comigrating with authentic T standard decreased over time in both medium and tissue samples. Radioactivity in the aqueous fraction remaining after ether extraction increased in a time-dependent manner, suggesting the presence of water-soluble conjugated steroids. Acid hydrolysis of the aqueous fraction yielded radioactivity that principally comigrated with T standard following a second ether extraction and TLC, along with a small, unidentified peak of poorly migrating radioactivity. Aromatization of T was assessed after charcoal stripping samples and measuring radioactivity remaining in the form of 3 H 2 O . Small but significant amounts of 3 H 2 O were present in both incubation medium and tissue samples. Incubation with the aromatase inhibitor 4-androsten-4-ol-3,17-dione acetate (ATD) decreased the formation of 3 H 2 O in a dose-dependent manner. These results suggest that goldfish mesenteric adipose tissue is capable of converting T to several metabolites including water-soluble conjugates and estrogen. |
| Databáze: | OpenAIRE |
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