Multi-metal tolerance of DHHC palmitoyl transferase-like protein isolated from metal contaminated soil
| Autor: | Bharti Thakur, Laurence Fraissinet-Tachet, Arkadeep Mukherjee, Roland Marmeisse, Ajay Kumar Pandey, M. Sudhakara Reddy |
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| Přispěvatelé: | Department of Biotechnology, Thapar Institute of Engineering and Technology, Department of Biotechnology, National Agri-Food Biotechnology Institute, Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Council of Scientific & Industrial Research (CSIR) - India Council of Scientific & Industrial Research (CSIR) - India38(1425)/16/EMR-IIFrench National Research Agency (ANR)4709-1 |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Sequence analysis Health Toxicology and Mutagenesis Mutant Yeast complementation 010501 environmental sciences Management Monitoring Policy and Law Toxicology 01 natural sciences Soil Protein-fragment complementation assay Transferases Complementary DNA Zinc finger Metals Heavy Transferase Soil Pollutants Gene 0105 earth and related environmental sciences Metatranscriptomics cDNA library Chemistry General Medicine Yeast Metal tolerance 010602 entomology Biochemistry [SDE]Environmental Sciences Palmitoyl transferase Cadmium |
| Zdroj: | Ecotoxicology Ecotoxicology, Springer Verlag, 2021, 30 (1), pp.67-79. ⟨10.1007/s10646-020-02301-5⟩ |
| ISSN: | 1573-3017 0963-9292 |
| DOI: | 10.1007/s10646-020-02301-5⟩ |
| Popis: | International audience; The microbiota inhabiting in metal polluted environment develops strong defense mechanisms to combat pollution and sustain life. Investigating the functional genes of the eukaryotic microbiota inhabiting in these environments by using metatranscriptomics approach was the focus of this study. Size fractionated eukaryotic cDNA libraries (library A, < 0.5 kb, library B, 0.5-1.0 kb, and library C, > 1.0 kb) were constructed from RNA isolated from the metal contaminated soil. The library C was screened for Cadmium (Cd) tolerant genes by using Cd sensitive yeast mutant ycf1(Delta) by functional complementation assay, which yielded various clones capable of growing in Cd amended media. One of the Cd tolerant clones, PLCg39 was selected because of its ability to grow at high concentrations of Cd. Sequence analysis of PLCg39 showed homology with DHHC palmitoyl transferases, which are responsible for addition of palmitoyl groups to proteins and usually possess metal coordination domains. The cDNA PLCg39 was able to confer tolerance to Cd-sensitive (ycf1(Delta)), Copper-sensitive (cup1(Delta)) and Zn-sensitive (zrc1(Delta)) yeast mutants when grown at different concentrations of Cd (40-100 mu M), Cu (150-1000 mu M) and Zn (10-13 mM), respectively. The DHHC mutant akr1(Delta) transformed with PLCg39 rescued from the metal sensitivity indicating the role of DHHC palmitoyl transferase in metal tolerance. This study demonstrated that PLCg39 acts as a potential metal tolerant gene which could be used in bioremediation, biosensing and other biotechnological fields. |
| Databáze: | OpenAIRE |
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