Altered thyroid status affects myocardial expression of connexin-43 and susceptibility of rat heart to malignant arrhythmias that can be partially normalized by red palm oil intake
| Autor: | Jitka Žurmanová, Narcisa Tribulova, Barbara Szeiffova Bacova, Vladimir Knezl, Tamara Egan Beňová, Dita Kasparova, Tomáš Soukup, Csilla Vinczenzová, Stanislav Pavelka |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Histology Thyroid Gland Connexin Administration Oral 030204 cardiovascular system & hematology Palm Oil Protein kinase C signaling 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Animals Plant Oils Euthyroid RNA Messenger Rats Wistar Molecular Biology Protein kinase C Messenger RNA business.industry Myocardium Thyroid Arrhythmias Cardiac Heart Cell Biology medicine.disease Rats Medical Laboratory Technology 030104 developmental biology Endocrinology medicine.anatomical_structure Connexin 43 Ventricular fibrillation cardiovascular system Phosphorylation Female sense organs business |
| Zdroj: | Histochemistry and cell biology. 147(1) |
| ISSN: | 1432-119X |
| Popis: | We aimed to study the impact of altered thyroid status on myocardial expression of electrical coupling protein connexin-43 (Cx43), the susceptibility of rats to ventricular fibrillation (VF) and the effects of antioxidant-rich red palm oil (RPO). Adult male and female euthyroid, hyperthyroid (treated with T3/T4), hypothyroid (treated with methimazole) Wistar rats supplemented or not with RPO for 6 weeks were used. Function of isolated perfused heart and VF threshold were determined. Left ventricular tissue was used for assessment of mRNA and protein levels of Cx43, its phosphorylated forms and topology. Protein kinase C signaling (PKC) and gene transcripts of some proteins related to cardiac arrhythmias were assessed. Hyperthyroid state resulted in decrease of total and phosphorylated forms of Cx43 and suppression of PKC-e expression in males and females, decrease of Cx43 mRNA in females, decrease of VF threshold and increase of functional parameters in male rat hearts. In contrast, hypothyroid status resulted in the increase of total and phosphorylated forms of Cx43, enhancement PKC-e expression in males and females, increase of Cx43 mRNA in females, increase of VF threshold and decrease of functional parameters in male rat hearts. Function of the heart was partially normalized by RPO intake, which also enhanced myocardial Cx43 and PKC-e expression as well as increased VF threshold in hyperthyroid male rats. We conclude that there is an inverse relationship between myocardial expression of Cx43, including its functional phosphorylated forms, and susceptibility of male rat hearts to VF in condition of altered thyroid status. RPO intake partly ameliorated adverse changes caused by excess of thyroid hormones. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink