Surface Modification To Reduce Nonspecific Binding of Quantum Dots in Live Cell Assays
| Autor: | Elizabeth L. Bentzen, Elaine Sanders-Bush, John N. Mason, Ian D. Tomlinson, Sandra J. Rosenthal, Paul J. Gresch, Michael R. Warnement, Randy D. Blakely, David W. Wright |
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| Rok vydání: | 2005 |
| Předmět: |
Cell type
Surface Properties Cytological Techniques Cell Acrylic Resins Biomedical Engineering Pharmaceutical Science Bioengineering Cell Line Polyethylene Glycols chemistry.chemical_compound Quantum Dots PEG ratio medicine Animals Binding site Fluorescent Dyes Pharmacology Binding Sites Organic Chemistry technology industry and agriculture medicine.anatomical_structure chemistry Biochemistry Quantum dot Cell culture Surface modification Ethylene glycol Biotechnology |
| Zdroj: | Bioconjugate Chemistry. 16:1488-1494 |
| ISSN: | 1520-4812 1043-1802 |
| DOI: | 10.1021/bc0502006 |
| Popis: | Nonspecific binding is a frequently encountered problem with fluorescent labeling of tissue cultures when labeled with quantum dots. In these studies various cell lines were examined for nonspecific binding. Evidence suggests that nonspecific binding is related to cell type and may be significantly reduced by functionalizing quantum dots with poly(ethylene glycol) ligands (PEG). The length of PEG required to give a significant reduction in nonspecific binding may be as short as 12-14 ethylene glycol units. |
| Databáze: | OpenAIRE |
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