Analysis of the frequency of single nucleotide polymorphisms in cytokine genes in patients with New Onset Diabetes After Transplant
| Autor: | Parasad Nair, Parul Aggarwal, Philip Chu, Tarek Mahmoud, Nashwa Othman, Torki Al-Otaibi, Mohamed Jahromi, Osama Gheith, Jamil Azzi, Medhat A-Halim, Sacha A. De Serres, Grace Messenger |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Male 0301 basic medicine Science Adaptive immunity 030232 urology & nephrology Single-nucleotide polymorphism Polymorphism Single Nucleotide Article 03 medical and health sciences Diabetes mellitus genetics 0302 clinical medicine Diabetes complications Interferon Diabetes mellitus Genotype Diabetes Mellitus medicine Humans Interferon gamma Kidney transplantation Multidisciplinary business.industry Middle Aged medicine.disease Kidney Transplantation Transplantation 030104 developmental biology Immunology Cytokines Medicine Female business medicine.drug |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | New Onset Diabetes After Transplantation (NODAT) is a serious metabolic complication. While β-cell dysfunction is considered the main contributing factor in the development of NODAT, the precise pathogenesis is not well understood. Cytokines are thought to be involved in the inflammation of islet β-cells in diabetes; however, few studies have investigated this hypothesis in NODAT. A total of 309 kidney transplant recipients (KTRs) were included in this study. An association between kidney transplants, and the development of diabetes after transplant (NODAT) was investigated. Comparison was made between KTRs who develop diabetes (NODAT cases) or did not develop diabetes (control), using key cytokines, IL-6 G (− 174)C, macrophage mediator; IL-4 C (− 490)T, T helper (Th)-2 cytokine profile initiator; Th-1 cytokine profile initiator interferon-γ T (+ 874) A gene and TGF β1 C (+ 869) T gene polymorphisms were investigated. The genes were amplified using well-established polymerase chain reaction (PCR) techniques in our laboratory. Compared to the AA and AT genotypes of interferon gamma (IFNG), there was a strong association between the TT genotype of IFNG and NODAT kidney transplant recipients (KTRs) versus non-NODAT KTRs (p = 0.005). The AA genotype of IFNG was found to be predominant in the control group (p = 0.004). Also, significant variations of IL6 G (− 174) C, IL-4 C (− 590) T, interferon-γ T (+ 874) A gene and transforming growth factor β1 C (+ 869) T may contribute to NODAT. Our data is consistent with theTh-1/T-reg pathway of immunity. Further larger pan Arab studies are required to confirm our findings. |
| Databáze: | OpenAIRE |
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